Polysaccharides derived from Shenling Baizhu San improve colitis via modulating tryptophan metabolism in mice.

Autor: Lv WJ; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Ma YM; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Huang JY; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., He SQ; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Li SP; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Lin J; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Chen R; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Lun JC; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China., Liu J; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China. Electronic address: 154364484@qq.com., Guo SN; College of Veterinary Medicine/Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, PR China; Guangdong Technology Research for Traditional Chinese Veterinary Medicine and Natural Medicine, Guangzhou, PR China. Electronic address: shining@scau.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2022 Dec 01; Vol. 222 (Pt A), pp. 1127-1136. Date of Electronic Publication: 2022 Oct 01.
DOI: 10.1016/j.ijbiomac.2022.09.246
Abstrakt: Shenling Baizhu San has beneficial effects on the metabolism of the gut microbiota, however, the mechanisms underlying microbiota metabolites mediated anti-inflammation signaling are not well understood. Previously, we have demonstrated that supplementation with Shenling Baizhu San alleviated antibiotic-associated diarrhea (AAD). The current study intends to investigate the dynamic modulation of Shenling Baizhu San polysaccharides (SP) on colitis from the gut microbiota metabolites perspective. Administration of SP effectively relieved colitis induced by DSS in mice, including alleviating body weight loss, the downregulation of colon proinflammatory mediators, and the promotion of intestinal injury repair. Whereas, the efficacy was eliminated by antibiotics, which demonstrated that the efficacy of SP was dependent on the gut microbiota. Fecal microbiota transplantation (FMT) showed that the efficacy of SP can be transferred to gut microbiota. Serum metabolomics analysis showed that supplementation with SP significantly promoted tryptophan metabolism, which was consistent with the changed structure of the gut microbiota, including Bacteroides, Bifidobacterium and Ruminococcus regulated by SP. Especially, the tryptophan metabolites-kynurenine (KYN) activated the expression of amplifying aryl-hydrocarbon receptor (AhR) and Cyp1A1 to promote IL-10 expression in colon. These data suggested that SP positively affected colitis in mice by regulating tryptophan metabolic function of their gut microbiota.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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