Determining Toxic Potencies of Water-Soluble Contaminants in Wastewater Influents and Effluent Using Gene Expression Profiling in C. elegans as a Bioanalytical Tool.

Autor: Karengera A; Department of Animal Sciences, Marine Animal Ecology Group, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA, Leeuwarden, The Netherlands., Verburg I; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA, Leeuwarden, The Netherlands.; Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, 9713 GZ, Groningen, The Netherlands., Sterken MG; Plant Sciences, Laboratory of Nematology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands., Riksen JAG; Plant Sciences, Laboratory of Nematology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands., Murk AJ; Department of Animal Sciences, Marine Animal Ecology Group, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands. tinka.murk@wur.nl., Dinkla IJT; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA, Leeuwarden, The Netherlands.
Jazyk: angličtina
Zdroj: Archives of environmental contamination and toxicology [Arch Environ Contam Toxicol] 2022 Oct; Vol. 83 (3), pp. 284-294. Date of Electronic Publication: 2022 Oct 03.
DOI: 10.1007/s00244-022-00959-y
Abstrakt: With chemical analysis, it is impossible to qualify and quantify the toxic potency of especially hydrophilic bioactive contaminants. In this study, we applied the nematode C. elegans as a model organism for detecting the toxic potency of whole influent wastewater samples. Gene expression in the nematode was used as bioanalytical tool to reveal the presence, type and potency of molecular pathways induced by 24-h exposure to wastewater from a hospital (H), nursing home (N), community (C), and influent (I) and treated effluent (E) from a local wastewater treatment plant. Exposure to influent water significantly altered expression of 464 genes, while only two genes were differentially expressed in nematodes treated with effluent. This indicates a significant decrease in bioactive pollutant-load after wastewater treatment. Surface water receiving the effluent did not induce any genes in exposed nematodes. A subset of 209 genes was differentially expressed in all untreated wastewaters, including cytochromes P450 and C-type lectins related to the nematode's xenobiotic metabolism and immune response, respectively. Different subsets of genes responded to particular waste streams making them candidates to fingerprint-specific wastewater sources. This study shows that gene expression profiling in C. elegans can be used for mechanism-based identification of hydrophilic bioactive compounds and fingerprinting of specific wastewaters. More comprehensive than with chemical analysis, it can demonstrate the effective overall removal of bioactive compounds through wastewater treatment. This bioanalytical tool can also be applied in the process of identification of the bioactive compounds via a process of toxicity identification evaluation.
(© 2022. The Author(s).)
Databáze: MEDLINE
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