A high-resolution map of small-scale inversions in the gibbon genome.

Autor:	Mercuri L; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy., Palmisano D; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy., L'Abbate A; IBIOM, Institute of Biomembranes, Bioenergetics, and Molecular Biotechnology, Bari 70125, Italy., D'Addabbo P; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy., Montinaro F; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy.; Estonian Biocentre, Institute of Genomics, University of Tartu, 51010 Tartu, Estonia., Catacchio CR; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy., Hasenfeld P; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany., Ventura M; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy., Korbel JO; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany., Sanders AD; Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 10115 Berlin, Germany.; Berlin Institute of Health (BIH), 10178 Berlin, Germany.; Charité-Universitätsmedizin, 10117 Berlin, Germany., Maggiolini FAM; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy.; Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria-Centro di Ricerca Viticoltura ed Enologia (CREA-VE), Turi 70010, Italy., Antonacci F; Dipartimento di Biologia, Università degli Studi di Bari 'Aldo Moro', Bari 70125, Italy.
Jazyk:	angličtina
Zdroj:	Genome research [Genome Res] 2022 Oct; Vol. 32 (10), pp. 1941-1951. Date of Electronic Publication: 2022 Sep 30.
DOI:	10.1101/gr.276960.122
Abstrakt:	Gibbons are the most speciose family of living apes, characterized by a diverse chromosome number and rapid rate of large-scale rearrangements. Here we performed single-cell template strand sequencing (Strand-seq), molecular cytogenetics, and deep in silico analysis of a southern white-cheeked gibbon genome, providing the first comprehensive map of 238 previously hidden small-scale inversions. We determined that more than half are gibbon specific, at least fivefold higher than shown for other primate lineage-specific inversions, with a significantly high number of small heterozygous inversions, suggesting that accelerated evolution of inversions may have played a role in the high sympatric diversity of gibbons. Although the precise mechanisms underlying these inversions are not yet understood, it is clear that segmental duplication-mediated NAHR only accounts for a small fraction of events. Several genomic features, including gene density and repeat (e.g., LINE-1) content, might render these regions more break-prone and susceptible to inversion formation. In the attempt to characterize interspecific variation between southern and northern white-cheeked gibbons, we identify several large assembly errors in the current GGSC Nleu3.0/nomLeu3 reference genome comprising more than 49 megabases of DNA. Finally, we provide a list of 182 candidate genes potentially involved in gibbon diversification and speciation. (© 2022 Mercuri et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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