Morphology and immunolocalization of intertubular steroidogenic cell in mesonephros of Podocnemis expansa during gonadal differentiation.

Autor:	de Macêdo LB; Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Magalhães MDS; Departamento de Morfologia, Universidade Federal do Amazonas, Manaus, AM, Brasil.; Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil., Dias LC; Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil., Lemos KKA; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., Borges RSM; Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Saraiva MVA; Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., de Oliveira MF; Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., de Assis AC; Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil., de Moura CEB; Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.
Jazyk:	angličtina
Zdroj:	Animal reproduction [Anim Reprod] 2022 Sep 05; Vol. 19 (3), pp. e20220011. Date of Electronic Publication: 2022 Sep 05 (Print Publication: 2022).
DOI:	10.1590/1984-3143-AR2022-0011
Abstrakt:	Sex steroid hormones are critical in gonadal differentiation in turtles. The gonads are not the only organs responsible for producing these hormones during this phase. Mesonephros play an important role in steroidogenesis. The present study aimed to investigate the presence of steroidogenic cells in mesonephros of Podocnemis expansa during gonadal differentiation and to evaluate their morphology and ultrastructure. Ten embryos of P. expansa were collected from 5 nests on day 36 of incubation, during spawning period on an artificial beach. Embryos were extracted from eggs by slicing the shell and euthanized. They were dissected under a stereoscopic microscope to collect the gonad-mesonephro complex, in which were fixed and subsequently processed for light microscopy, immunohistochemistry and transmission electron microscopy analysis. During histological analysis was observed mesonephros has typical morphological structure. Immunohistochemistry showed immunoreaction to aromatase in cells of intertubular space. Confirming these findings, it was possible to observe a type of intertubular cell in several regions of mesonephro, being more predominant in region close to blood vessels, distal and proximal tubules. In ultrastructural analysis these cells were characterized by having a clear, large, and rounded nucleus with evident nucleolus and cytoplasm rich in electron-dense droplets. This study demonstrated for the first time the presence of cells with morphological, immunohistochemical and ultrastructural characteristics similar to steroid-producing cells in P. expansa mesonephrons, suggesting that this organ may contribute to gonadal differentiation in this species. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
Databáze:	MEDLINE
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