Fluorimetric Assay of FAAH Activity.
| Autor: | Angelucci CB; Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. bcangelucci@unite.it., Giacominelli-Stuffler R; Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy., Maccarrone M; Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.; European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2576, pp. 249-260. |
| DOI: | 10.1007/978-1-0716-2728-0_21 |
| Abstrakt: | Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid (AA) and ethanolamine. The method described here measures FAAH activity through the fluorometric arachidonoyl-7-amino-4-methyl-coumarin amide (AAMCA) substrate, which allows a simple and sensitive assay suitable for high-throughput screening tests. FAAH catalyzes the hydrolysis of AAMCA producing AA and the highly fluorescent compound 7-amino-4-methylcoumarin (AMC). (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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