Phage-mimicking antibacterial core-shell nanoparticles.

Autor:	Hopf J; Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame Notre Dame IN USA., Waters M; Department of Aerospace and Mechanical Engineering, University of Notre Dame Notre Dame IN USA pnallath@nd.edu +1 574 631 7868., Kalwajtys V; Department of Biological Sciences, University of Notre Dame Notre Dame IN USA., Carothers KE; Department of Biological Sciences, University of Notre Dame Notre Dame IN USA., Roeder RK; Department of Aerospace and Mechanical Engineering, University of Notre Dame Notre Dame IN USA pnallath@nd.edu +1 574 631 7868.; Center for Nanoscience and Technology (NDnano), University of Notre Dame Notre Dame IN USA., Shrout JD; Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame Notre Dame IN USA., Lee SW; Department of Biological Sciences, University of Notre Dame Notre Dame IN USA., Nallathamby PD; Department of Aerospace and Mechanical Engineering, University of Notre Dame Notre Dame IN USA pnallath@nd.edu +1 574 631 7868.; Center for Nanoscience and Technology (NDnano), University of Notre Dame Notre Dame IN USA.; Center for Advanced Diagnostics and Therapeutics (AD&T), University of Notre Dame Notre Dame IN USA.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2019 Nov 07; Vol. 1 (12), pp. 4812-4826. Date of Electronic Publication: 2019 Nov 07 (Print Publication: 2019).
DOI:	10.1039/c9na00461k
Abstrakt:	The increasing frequency of nosocomial infections caused by antibiotic-resistant microorganisms concurrent with the stagnant discovery of new classes of antibiotics has made the development of new antibacterial agents a critical priority. Our approach is an antibiotic-free strategy drawing inspiration from bacteriophages to combat antibiotic-resistant bacteria. We developed a nanoparticle-based antibacterial system that structurally mimics the protein-turret distribution on the head structure of certain bacteriophages and explored a combination of different materials arranged hierarchically to inhibit bacterial growth and ultimately kill pathogenic bacteria. Here, we describe the synthesis of phage-mimicking antibacterial nanoparticles (ANPs) consisting of silver-coated gold nanospheres distributed randomly on a silica core. The silver-coating was deposited in an anisotropic fashion on the gold nanospheres. Structurally, our nanoparticles mimicked the bacteriophages of the family Microviridae by up to 88%. These phage-mimicking ANPs were tested for bactericidal efficacy against four clinically relevant nosocomial pathogens ( Staphylococcus aureus USA300, Pseudomonas aeruginosa FRD1, Enterococcus faecalis , and Corynebacterium striatum ) and for biocompatibility with skin cells. Bacterial growth of all four bacteria was inhibited (21% to 90%) as well as delayed (by up to 5 h). The Gram-positive organisms were shown to be more sensitive to the nanoparticle treatment. Importantly, the phage-mimicking ANPs did not show any significant cytotoxic effects against human skin keratinocytes. Our results indicate the potential for phage-mimicking antimicrobial nanoparticles as a highly effective, alternative antibacterial agent, which may be suitable for co-administration with existing available formulations. Competing Interests: There are no conflicts of interests to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu