An intelligent DNA nanorobot for detection of MiRNAs cancer biomarkers using molecular programming to fabricate a logic-responsive hybrid nanostructure.

Autor: Mirzaiebadizi A; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.; Institute of Biochemistry and Molecular Biology II, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany., Ravan H; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran. ravan@uk.ac.ir., Dabiri S; Department of Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran. sh_dabiri@kmu.ac.ir., Mohammadi P; Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran., Shahba A; Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Ziasistani M; Department of Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran., Khatami M; Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2022 Nov; Vol. 45 (11), pp. 1781-1797. Date of Electronic Publication: 2022 Sep 20.
DOI: 10.1007/s00449-022-02785-x
Abstrakt: Herein, we designed a DNA framework-based intelligent nanorobot using toehold-mediated strand displacement reaction-based molecular programming and logic gate operation for the selective and synchronous detection of miR21 and miR125b, which are known as significant cancer biomarkers. Moreover, to investigate the applicability of our design, DNA nanorobots were implemented as capping agents onto the pores of MSNs. These agents can develop a logic-responsive hybrid nanostructure capable of specific drug release in the presence of both targets. The prosperous synthesis steps were verified by FTIR, XRD, BET, UV-visible, FESEM-EDX mapping, and HRTEM analyses. Finally, the proper release of the drug in the presence of both target microRNAs was studied. This Hybrid DNA Nanostructure was designed with the possibility to respond to any target oligonucleotides with 22 nucleotides length.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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