SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived Cells.

Autor:	Nguyen LC; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Renner DM; Department of Microbiology, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA.; Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA., Silva D; Department of Pathology, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Yang D; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Parenti NA; Department of Microbiology, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA.; Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA., Medina KM; Department of Microbiology, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA., Nicolaescu V; Department of Microbiology, University of Chicagogrid.170205.1, Chicago, Illinois, USA.; Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois, USA., Gula H; Department of Microbiology, University of Chicagogrid.170205.1, Chicago, Illinois, USA.; Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois, USA., Drayman N; Pritzker School of Molecular Engineering, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Valdespino A; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Mohamed A; Pritzker School of Molecular Engineering, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Dann C; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Wannemo K; Department of Pathology, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Robinson-Mailman L; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Gonzalez A; Department of Pathology, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Stock L; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Cao M; Department of Pathology, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Qiao Z; Department of Chemistry, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Moellering RE; Department of Chemistry, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Tay S; Pritzker School of Molecular Engineering, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Randall G; Department of Microbiology, University of Chicagogrid.170205.1, Chicago, Illinois, USA.; Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois, USA., Beers MF; Department of Medicine, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA.; Penn-CHOP Lung Biology Institute, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA., Rosner MR; Ben May Department for Cancer Research, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Oakes SA; Department of Pathology, University of Chicagogrid.170205.1, Chicago, Illinois, USA., Weiss SR; Department of Microbiology, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA.; Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvaniagrid.25879.31, Philadelphia, Pennsylvania, USA.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2022 Oct 26; Vol. 13 (5), pp. e0241522. Date of Electronic Publication: 2022 Sep 20.
DOI:	10.1128/mbio.02415-22
Abstrakt:	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed over 6 million individuals worldwide and continues to spread in countries where vaccines are not yet widely available or its citizens are hesitant to become vaccinated. Therefore, it is critical to unravel the molecular mechanisms that allow SARS-CoV-2 and other coronaviruses to infect and overtake the host machinery of human cells. Coronavirus replication triggers endoplasmic reticulum (ER) stress and activation of the unfolded protein response (UPR), a key host cell pathway widely believed to be essential for viral replication. We examined the master UPR sensor IRE1α kinase/RNase and its downstream transcription factor effector XBP1s, which is processed through an IRE1α-mediated mRNA splicing event, in human lung-derived cells infected with betacoronaviruses. We found that human respiratory coronavirus OC43 (HCoV-OC43), Middle East respiratory syndrome coronavirus (MERS-CoV), and murine coronavirus (MHV) all induce ER stress and strongly trigger the kinase and RNase activities of IRE1α as well as XBP1 splicing. In contrast, SARS-CoV-2 only partially activates IRE1α through autophosphorylation, but its RNase activity fails to splice XBP1. Moreover, while IRE1α was dispensable for replication in human cells for all coronaviruses tested, it was required for maximal expression of genes associated with several key cellular functions, including the interferon signaling pathway, during SARS-CoV-2 infection. Our data suggest that SARS-CoV-2 actively inhibits the RNase of autophosphorylated IRE1α, perhaps as a strategy to eliminate detection by the host immune system. IMPORTANCE SARS-CoV-2 is the third lethal respiratory coronavirus, after MERS-CoV and SARS-CoV, to emerge this century, causing millions of deaths worldwide. Other common coronaviruses such as HCoV-OC43 cause less severe respiratory disease. Thus, it is imperative to understand the similarities and differences among these viruses in how each interacts with host cells. We focused here on the inositol-requiring enzyme 1α (IRE1α) pathway, part of the host unfolded protein response to virus-induced stress. We found that while MERS-CoV and HCoV-OC43 fully activate the IRE1α kinase and RNase activities, SARS-CoV-2 only partially activates IRE1α, promoting its kinase activity but not RNase activity. Based on IRE1α-dependent gene expression changes during infection, we propose that SARS-CoV-2 prevents IRE1α RNase activation as a strategy to limit detection by the host immune system.
Databáze:	MEDLINE
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