CircFBXW7 Inhibits Proliferation, Migration, and Invasion of Nonsmall Cell Lung Cancer Cells by Regulating miR-492.

Autor:	Zhu SY; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Zhang ZX; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Gu L; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Rao DY; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Tang XY; Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Sang CP; Gannan Medical University, Ganzhou 341000, China., Xia Y; Gannan Medical University, Ganzhou 341000, China., Si MY; Gannan Medical University, Ganzhou 341000, China., Shi HQ; Department of Oncology, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Li J; Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Li SL; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Xie CF; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Yuan XL; Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China., Tang ZX; Department of Thoracic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.
Jazyk:	angličtina
Zdroj:	Journal of oncology [J Oncol] 2022 Sep 08; Vol. 2022, pp. 8699359. Date of Electronic Publication: 2022 Sep 08 (Print Publication: 2022).
DOI:	10.1155/2022/8699359
Abstrakt:	Background: CircFBXW7 has been determined to be involved in various cancers; however, its role in nonsmall cell lung cancer (NSCLC) remains unclear. This study examined the function and potential mechanism of circFBXW7 in NSCLC. Methods: The structure of circFBXW7 was verified via RT-PCR and Sanger sequencing. The expression of circFBXW7 in NSCLC was determined by qRT-PCR. The effect of circFBXW7 overexpression on the proliferation, migration, and invasion of NSCLC cells was examined by CCK-8 and Transwell assays. Furthermore, a circFBXW7-miRNA network was established to explore their interaction. Predicted miRNA was determined by qRT-PCR. Moreover, the miRNA mimics were synthesized, wherein its effect on proliferation, migration, and invasion of NSCLC cells overexpressed circFBXW7 was assessed. Results: The circularity of circFBXW7 was verified. The expression of circFBXW7 was found to be downregulated in NSCLC cells compared with that in normal human lung epithelial BEAS-2B cells. Overexpression of circFBXW7 reduced cell proliferation, migration, and invasion. Furthermore, according to the circFBXW7-miRNA network prediction and qRT-PCR validation, miR-492 was identified to be the target of circFBXW7. The inhibitory effect of circFBXW7 overexpression on cell proliferation, migration, and invasion was reversed by miR-492 mimics. Conclusion: CircFBXW7 is downregulated in NSCLC. CircFBXW7 inhibits NSCLC cells proliferation, migration, and invasion by regulating miR-492. Competing Interests: The authors declare that they have no conflicts of interest. (Copyright © 2022 Shen-Yu Zhu et al.)
Databáze:	MEDLINE
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