Evaluation of the oxytocin effect in a rat model with experimental periodontitis.

Autor: Paksoy T; Department of Periodontology, Faculty of Dentistry, İstanbul Atlas University, İstanbul, Turkey. tugceakap86@hotmail.com., Ustaoğlu G; Department of Periodontology, Gülhane Faculty of Dentistry, University of Health Sciences, Ankara, Turkey., Şehirli AÖ; Department of Pharmacology, Faculty of Dentistry, Near East University, Mersin 10, Turkey., Ünsal RBK; Department of Periodontology, Faculty of Dentistry, University of Kyrenia, Mersin 10, Turkey., Sayıner S; Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, Mersin 10, Turkey., Orhan K; Department of DentoMaxillofacial Radiology, Ankara University, Ankara, Turkey., Aycı NB; Department of Histology and Embryology, School of Medicine, Tekirdağ Namık Kemal University, Tekirdağ, Turkey., Çetinel Ş; Department of Histology and Embryology, School of Medicine, Marmara University, İstanbul, Turkey., Aksoy U; Department of Endodontics, Faculty of Dentistry, Near East University, Mersin 10, Turkey.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2022 Dec; Vol. 395 (12), pp. 1599-1608. Date of Electronic Publication: 2022 Sep 17.
DOI: 10.1007/s00210-022-02293-5
Abstrakt: The aim of the present study was to evaluate the inhibitory effects of oxytocin on the development of periodontitis based on its properties against bone loss and resorption. Thirty-two Wistar albino rats were divided into four equal groups: control, periodontitis + saline, periodontitis + 0.5 mg/kg/day oxytocin, and periodontitis + 1 mg/kg/day oxytocin. Periodontitis groups received 4.0 silk ligatures around their cervixes of the right and left mandibular incisors in an "8" shape, kept for 14 days. Animals in oxytocin groups were injected once every day during 14 days with oxytocin. The mandibles were fixed and scanned using microcomputed tomography to quantify bone resorption and volumetric measurements. Blood samples were collected to analyze the concentrations of macrophage colony-stimulating factor (M-CSF), receptor activator of nuclear factor-κΒ ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase-8 (MMP-8), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, glutathione peroxidase (GPx), superoxide dismutase (SOD), and malondialdehyde (MDA). Histopathological evaluations were conducted to examine the gingiva and alveolar bone. Oxytocin prevented the development of periodontitis by decreasing ligament deteriorations and leukocytes in the gingival connective tissue and promoting reintegration with the alveolar bone. Bone resorption in all regions was less in the periodontitis + 1 mg/kg/day oxytocin group than in the periodontitis + saline group. Although TNF-α, IL-6, and RANKL values were lower in the periodontitis + 1 mg/kg/day oxytocin group, OPG was higher than that in the periodontitis + saline group. M-CSF, MMP-8, and MDA were lower in the oxytocin groups than in the periodontitis + saline group. Oxytocin may be an effective agent for periodontal diseases because it decreased bone resorption, oxidative stress, and inflammation in an experimental periodontitis.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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