Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development.

Autor: Smith KA; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA., Lin AH; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA.; Scientific Computing and Imaging Institute, University of Utah, Salt Lake City, UT, 84112, USA., Stevens AH; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA., Yu SM; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA.; Department of Molecular Pharmaceutics, University of Utah, Salt Lake City, UT, 84112, USA., Weiss JA; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA.; Scientific Computing and Imaging Institute, University of Utah, Salt Lake City, UT, 84112, USA.; Department of Orthopaedics, University of Utah, Salt Lake City, UT, 84112, USA., Timmins LH; Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA. lucas.timmins@utah.edu.; Scientific Computing and Imaging Institute, University of Utah, Salt Lake City, UT, 84112, USA. lucas.timmins@utah.edu.
Jazyk: angličtina
Zdroj: Journal of cardiovascular translational research [J Cardiovasc Transl Res] 2023 Apr; Vol. 16 (2), pp. 463-472. Date of Electronic Publication: 2022 Sep 12.
DOI: 10.1007/s12265-022-10316-y
Abstrakt: Remodeling of extracellular matrix proteins underlies the development of cardiovascular disease. Herein, we utilized a novel molecular probe, collagen hybridizing peptide (CHP), to target collagen molecular damage during atherogenesis. The thoracic aorta was dissected from ApoE -/- mice that had been on a high-fat diet for 0-18 weeks. Using an optimized protocol, tissues were stained with Cy3-CHP and digested to quantify CHP with a microplate assay. Results demonstrated collagen molecular damage, inferred from Cy3-CHP fluorescence, was a function of location and time on the high-fat diet. Tissue from the aortic arch showed a significant increase in collagen molecular damage after 18 weeks, while no change was observed in tissue from the descending aorta. No spatial differences in fluorescence were observed between the superior and inferior arch tissue. Our results provide insight into the early changes in collagen during atherogenesis and present a new opportunity in the subclinical diagnosis of atherosclerosis.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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