Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice.

Autor: Morgan HL; Lifespan and Population Health, School of Medicine, University of Nottingham, Nottingham, NG7 2UH, UK., Furse S; Core Metabolomics and Lipidomics Laboratory, Wellcome-MRC Institute of Metabolic Science, University of Cambridge, Addenbrooke's Treatment Centre, Keith Day Road, Cambridge, CB2 0QQ, UK.; Wellcome-MRC Institute of Metabolic Science and Medical Research Council Metabolic Diseases Unit, University of Cambridge, Cambridge, CB2 0QQ, UK.; Biological Chemistry Group, Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, TW9 3AE, UK., Dias IHK; Aston Medical School, Aston University, Aston Triangle, Birmingham, B4 7ET, UK., Shabir K; Aston Medical School, Aston University, Aston Triangle, Birmingham, B4 7ET, UK., Castellanos M; Nottingham Arabidopsis Stock Centre (NASC), University of Nottingham, Sutton Bonington Campus, Plant Science Building, School of Biosciences, Loughborough, LE12 5RD, UK., Khan I; Nottingham Arabidopsis Stock Centre (NASC), University of Nottingham, Sutton Bonington Campus, Plant Science Building, School of Biosciences, Loughborough, LE12 5RD, UK., May ST; Nottingham Arabidopsis Stock Centre (NASC), University of Nottingham, Sutton Bonington Campus, Plant Science Building, School of Biosciences, Loughborough, LE12 5RD, UK., Holmes N; Deep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Carlile M; Deep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Sang F; Deep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Wright V; Deep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Koulman A; Core Metabolomics and Lipidomics Laboratory, Wellcome-MRC Institute of Metabolic Science, University of Cambridge, Addenbrooke's Treatment Centre, Keith Day Road, Cambridge, CB2 0QQ, UK.; Wellcome-MRC Institute of Metabolic Science and Medical Research Council Metabolic Diseases Unit, University of Cambridge, Cambridge, CB2 0QQ, UK., Watkins AJ; Lifespan and Population Health, School of Medicine, University of Nottingham, Nottingham, NG7 2UH, UK. adam.watkins@nottingham.ac.uk.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Sep 08; Vol. 5 (1), pp. 929. Date of Electronic Publication: 2022 Sep 08.
DOI: 10.1038/s42003-022-03914-8
Abstrakt: The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. Using artificial insemination, in combination with vasectomised male mating, we generated offspring using either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Offspring from either LPD sperm or seminal fluid display elevated body weight and tissue dyslipidaemia from just 3 weeks of age. These changes become more pronounced in adulthood, occurring in conjunction with altered hepatic metabolic and inflammatory pathway gene expression. Second generation offspring also display differential tissue lipid abundance, with profiles similar to those of first generation adults. These findings demonstrate that offspring metabolic homeostasis is perturbed in response to a suboptimal paternal diet with the effects still evident within a second generation.
(© 2022. The Author(s).)
Databáze: MEDLINE
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