| Autor: |
da Silva FR; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Soares Thimoteo D; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Ferraz Carbonel A; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil.; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Universidade Federal de São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Fuchs LFP; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., da Silva Sasso GR; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Universidade Federal de São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Dos Santos Simões R; Department of Obstetrics and Gynecology, Medicine Faculty of University of São Paulo - FMUSP, São Paulo, SP, Brazil., Invitti A; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Ramos Vieira R; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., de Souza Ferreira LP; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Universidade Federal de São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., da Silva RA; Biosciences Graduate Program, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (IBILCE/UNESP), São José do Rio Preto, SP, Brazil., Lima PDA; Queen's Cardiopulmonary Unit (QCPU), Queen's University, Kingston, ON, Canada., Soares Júnior JM; Department of Obstetrics and Gynecology, Medicine Faculty of University of São Paulo - FMUSP, São Paulo, SP, Brazil., de Jesus Simões M; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil.; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Universidade Federal de São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil., Bertoncini CRA; Department of Gynecology - Paulista School of Medicine, Federal University of São Paulo - EPM/UNIFESP, São Paulo, SP, Brazil. |
| Abstrakt: |
Objective: Evaluate histomorphometry of ectopic and eutopic endometrial tissues in receptor mice. Method: Eighteen female Balb/c were divided into 3 groups, 6 animals each: GI Control, no procedure; GII - Sham, animals that had the same procedures as GIII without receiving the ectopic endometrial implant. Instead, they received saline solution; GIII - endometriosis model, animals had surgical intervention with an ectopic endometrial implant. GI and GIII mice were treated with 17β-estradiol, 100 µg/kg each. All animals were euthanized to collect uterine horns, which were fixed in 4% paraformaldehyde, embedded in paraffin, stained with Hematoxilin and Eosin and submitted to histomorphometric analyzes. Data underwent one-way ANOVA followed by Tukey's test. Results: Local tissue growth, showing important lesions and adhesions, as well as dark cysts were noticed. In GIII group, there was an increase in number of blood vessels and glands (GIII ≥ GI and GIII p > .001). Thickening of the GIII endometrial epithelial was also evident (GIII ≥ GI and GIII. p > .001). We also noticed an increase in the number of eosinophils (GIII (GIII ≥ GI and GIII. p > .001). Conclusion: Easy to perform model, capable of reproducing morphological endometriosis characteristics. From our findings, there was an increase of endometrial thickness as well as an increase in the eosinophils population. |
