| Autor: |
Sherbo RS; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.; Department of Systems Biology, Harvard Medical School, Boston, MA 02115., Silver PA; Department of Systems Biology, Harvard Medical School, Boston, MA 02115., Nocera DG; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138. |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Sep 13; Vol. 119 (37), pp. e2210538119. Date of Electronic Publication: 2022 Sep 06. |
| DOI: |
10.1073/pnas.2210538119 |
| Abstrakt: |
Microbes can provide a more sustainable and energy-efficient method of food and nutrient production compared to plant and animal sources, but energy-intensive carbon (e.g., sugars) and nitrogen (e.g., ammonia) inputs are required. Gas-fixing microorganisms that can grow on H 2 from renewable water splitting and gaseous CO 2 and N 2 offer a renewable path to overcoming these limitations but confront challenges owing to the scarcity of genetic engineering in such organisms. Here, we demonstrate that the hydrogen-oxidizing carbon- and nitrogen-fixing microorganism Xanthobacter autotrophicus grown on a CO 2 /N 2 /H 2 gas mixture can overproduce the vitamin riboflavin (vitamin B 2 ). We identify plasmids and promoters for use in this bacterium and employ a constitutive promoter to overexpress riboflavin pathway enzymes. Riboflavin production is quantified at 15 times that of the wild-type organism. We demonstrate that riboflavin overproduction is maintained when the bacterium is grown under hybrid inorganic-biological conditions, in which H 2 from water splitting, along with CO 2 and N 2 , is fed to the bacterium, establishing the viability of the approach to sustainably produce food and nutrients. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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