Morphofunctional characterization of hemocytes in black soldier fly larvae.

Autor: Bruno D; Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy., Montali A; Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy., Gariboldi M; Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy., Wrońska AK; Host Parasites Molecular Interaction Research Unit, Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland., Kaczmarek A; Host Parasites Molecular Interaction Research Unit, Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland., Mohamed A; Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt., Tian L; Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, Guangdong Provincial Sericulture and Mulberry Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, China., Casartelli M; Department of Biosciences, University of Milano, Milano, Italy.; Interuniversity Center for Studies on Bioinspired Agro-environmental Technology (BAT Center), University of Napoli Federico II, Portici, Italy., Tettamanti G; Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.; Interuniversity Center for Studies on Bioinspired Agro-environmental Technology (BAT Center), University of Napoli Federico II, Portici, Italy.
Jazyk: angličtina
Zdroj: Insect science [Insect Sci] 2023 Aug; Vol. 30 (4), pp. 912-932. Date of Electronic Publication: 2022 Oct 06.
DOI: 10.1111/1744-7917.13111
Abstrakt: In insects, the cell-mediated immune response involves an active role of hemocytes in phagocytosis, nodulation, and encapsulation. Although these processes have been well documented in multiple species belonging to different insect orders, information concerning the immune response, particularly the hemocyte types and their specific function in the black soldier fly Hermetia illucens, is still limited. This is a serious gap in knowledge given the high economic relevance of H. illucens larvae in waste management strategies and considering that the saprophagous feeding habits of this dipteran species have likely shaped its immune system to efficiently respond to infections. The present study represents the first detailed characterization of black soldier fly hemocytes and provides new insights into the cell-mediated immune response of this insect. In particular, in addition to prohemocytes, we identified five hemocyte types that mount the immune response in the larva, and analyzed their behavior, role, and morphofunctional changes in response to bacterial infection and injection of chromatographic beads. Our results demonstrate that the circulating phagocytes in black soldier fly larvae are plasmatocytes. These cells also take part in nodulation and encapsulation with granulocytes and lamellocyte-like cells, developing a starting core for nodule/capsule formation to remove/encapsulate large bacterial aggregates/pathogens from the hemolymph, respectively. These processes are supported by the release of melanin precursors from crystal cells and likely by mobilizing nutrient reserves in newly circulating adipohemocytes, which could thus trophically support other hemocytes during the immune response. Finally, the regulation of the cell-mediated immune response by eicosanoids was investigated.
(© 2022 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje