TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.

Autor: Brenner JL; Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA., Jyo EM; Department of Biology, Syracuse University, Syracuse, NY, 13210, USA., Mohammad A; Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA., Fox P; Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA., Jones V; Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA., Mardis E; McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, 63110, USA., Schedl T; Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA. Electronic address: ts@wustl.edu., Maine EM; Department of Biology, Syracuse University, Syracuse, NY, 13210, USA. Electronic address: emmaine@syr.edu.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2022 Nov; Vol. 491, pp. 43-55. Date of Electronic Publication: 2022 Sep 03.
DOI: 10.1016/j.ydbio.2022.08.010
Abstrakt: Many tissues contain multipotent stem cells that are critical for maintaining tissue function. In Caenorhabditis elegans, germline stem cells allow gamete production to continue in adulthood. In the gonad, GLP-1/Notch signaling from the distal tip cell niche to neighboring germ cells activates a complex regulatory network to maintain a stem cell population. GLP-1/Notch signaling positively regulates production of LST-1 and SYGL-1 proteins that, in turn, interact with a set of PUF/FBF proteins to positively regulate the stem cell fate. We previously described sog (suppressor of glp-1 loss of function) and teg (tumorous enhancer of glp-1 gain of function) genes that limit the stem cell fate and/or promote the meiotic fate. Here, we show that sog-10 is allelic to nhl-2. NHL-2 is a member of the conserved TRIM-NHL protein family whose members can bind RNA and ubiquitinate protein substrates. We show that NHL-2 acts, at least in part, by inhibiting the expression of PUF-3 and PUF-11 translational repressor proteins that promote the stem cell fate. Two other negative regulators of stem cell fate, CGH-1 (conserved germline helicase) and ALG-5 (Argonaute protein), may work with NHL-2 to modulate the stem cell population. In addition, NHL-2 activity promotes the male germ cell fate in XX animals.
Competing Interests: Declarations of competing interest None.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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