Effect of Conditioned Media of Limbal Epithelial Cells and Corneal Stromal Cells on Functional Activity of Limb Mesenchymal Stem Cells.

Autor: Bondarenko NA; Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. bond802888@yandex.ru., Surovtseva MA; Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Kim II; Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Lykov AP; Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Iskakov IA; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health - Novosibirsk Branch, Novosibirsk, Russia., Trunov AN; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health - Novosibirsk Branch, Novosibirsk, Russia., Chernykh VV; S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health - Novosibirsk Branch, Novosibirsk, Russia., Poveshchenko OV; Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Aug; Vol. 173 (4), pp. 464-467. Date of Electronic Publication: 2022 Sep 05.
DOI: 10.1007/s10517-022-05562-8
Abstrakt: We studied the effect of conditioned media from limbal epithelial stem cells, fibroblasts, and corneal keratocytes on the functional activity of human limbal mesenchymal stem cells. It was shown that the conditioned media from limbal epithelial stem cells reduced proliferative activity and inhibited migration of limbal mesenchymal stem cells. In the conditioned media of limbal epithelial stem cells, increased concentrations of VEGF and TNFα and reduced concentration of BDNF, vimentin, and fibronectin were found. The conditioned medium from corneal stromal cells did not affect functional activity of mesenchymal stem cells in the limbus. These data contribute to the understanding of the interaction of cells in the limbal niche and with corneal cells essential for the maintenance of the cellular homeostasis in the cornea.
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Databáze: MEDLINE
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