Structural changes of erythrocyte membrane revealed by 3D confocal optical profilometer.

Autor: Lomanovskaya TA; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Piavchenko GA; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Soldatov VO; Pharmacology and Clinical Pharmacology Department, Belgorod National Research University, Belgorod, Russia., Venediktov AA; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Kuzmin EA; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Kartashkina NL; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Mukhamedova SG; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Boronikhina TV; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Markov AG; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Telyshev DV; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.; Institute of Biomedical Systems, National Research University of Electronic Technology, Zelenograd, Moscow, Russia., Meglinski I; Opto-Electronics and Measurement Techniques, Faculty of Information and Electrical Engineering, University of Oulu, Oulu, Finland.; College of Engineering and Physical Sciences, Aston University, Birmingham, UK., Yatskovskiy AN; Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2023 Jan; Vol. 16 (1), pp. e202200222. Date of Electronic Publication: 2022 Sep 03.
DOI: 10.1002/jbio.202200222
Abstrakt: We examined hematological changes influenced by the experimental hypervitaminosis A. The 3D confocal optical profilometer was applied for assessment of the erythrocytes' membrane structural changes influenced by an overdose of vitamin A. The blood smears were evaluated in terms of alterations of geometrical and optical parameters of erythrocytes for two groups of animals: oil base and retinol palmitate (n = 9 animals for each group). The results demonstrate that an overdose of retinol palmitate causes changes in the torus curvature and pallor of discocytes, their surface area and volume. The observed structural malformations of the shape of red blood cells become visible at the earlier preclinical stage of changes in animal state and behavior. With this in mind, the results of the study open a new area of research in the certain dysfunction diagnosis of red blood cells and have a great potential in the further development of new curative protocols.
(© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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