Genetically diverse mouse platform to xenograft cancer cells.

Autor:	Sargent JK; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Warner MA; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Low BE; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Schott WH; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Hoffert T; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Coleman D; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Woo XY; The Jackson Laboratory for Genomic Medicine, 10 Discovery Drive, Farmington, CT 06032, USA., Sheridan T; The Jackson Laboratory for Genomic Medicine, 10 Discovery Drive, Farmington, CT 06032, USA.; Hartford Hospital, Department of Pathology, 80 Seymour Street, Hartford, CT 06102, USA., Erattupuzha S; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Henrich PP; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Philip VM; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Chuang JH; The Jackson Laboratory for Genomic Medicine, 10 Discovery Drive, Farmington, CT 06032, USA., Wiles MV; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA., Hasham MG; The Jackson Laboratory for Mouse Genetics, 600 Main Street, Bar Harbor, ME 04609, USA.
Jazyk:	angličtina
Zdroj:	Disease models & mechanisms [Dis Model Mech] 2022 Sep 01; Vol. 15 (9). Date of Electronic Publication: 2022 Aug 29.
DOI:	10.1242/dmm.049457
Abstrakt:	The lack of genetically diverse preclinical animal models in basic biology and efficacy testing has been cited as a potential cause of failure in clinical trials. We developed and characterized five diverse RAG1 null mouse strains as models that allow xenografts to grow. In these strains, we characterized the growth of breast cancer, leukemia and glioma cell lines. We found a wide range of growth characteristics that were far more dependent on strain than tumor type. For the breast cancer cell line, we characterized the spectrum of xenograft/tumor growth at structural, histological, cellular and molecular levels across each strain, and found that each strain captures unique structural components of the stroma. Furthermore, we showed that the increase in tumor-infiltrating myeloid CD45+ cells and the amount of circulating cytokine IL-6 and chemokine KC (also known as CXCL1) is associated with a higher tumor size in different strains. This resource is available to study established human xenografts, as well as difficult-to-xenograft tumors and growth of hematopoietic stems cells, and to decipher the role of myeloid cells in the development of spontaneous cancers. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu