Validation and clinical application of a method to quantify efavirenz in cervicovaginal secretions from flocked swabs using liquid chromatography tandem mass spectrometry.

Autor: Olagunju A; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK.; Department of Pharmaceutical Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria., Nwogu J; Department of Pharmaceutical Chemistry, University of Ibadan, Ibadan, Nigeria., Eniayewu O; Department of Pharmaceutical Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.; Department of Pharmaceutical and Medicinal Chemistry, University of Ilorin, Ilorin, Nigeria., Atoyebi S; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK.; Department of Pharmaceutical Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria., Amara A; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK., Kpamor J; Federal Medical Centre, Makurdi, Nigeria., Bolaji O; Department of Pharmaceutical Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria., Adejuyigbe E; Department of Paediatrics and Child Health, Obafemi Awolowo University, Ile-Ife, Nigeria., Owen A; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK., Khoo S; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK.
Jazyk: angličtina
Zdroj: Wellcome open research [Wellcome Open Res] 2022 Jul 08; Vol. 6, pp. 246. Date of Electronic Publication: 2022 Jul 08 (Print Publication: 2021).
DOI: 10.12688/wellcomeopenres.17202.3
Abstrakt: Background : A liquid chromatography tandem mass spectrometry method to quantify drugs in dried cervicovaginal secretions from flocked swabs was developed and validated using the antiretroviral efavirenz as an example. Methods: Cervicovaginal swabs (CVS) were prepared by submerging flocked swabs in efavirenz-spiked plasma matrix. Time to full saturation, weight uniformity, recovery and room temperature stability were evaluated. Chromatographic separation was on a reverse-phase C18 column by gradient elution using 1mM ammonium acetate in water/acetonitrile at 400 µL/min. Detection and quantification were on a TSQ Quantum Access triple quadrupole mass spectrometer operated in negative ionisation mode. The method was used to quantify efavirenz in CVS samples from human immunodeficiency virus (HIV)-positive women in the VADICT study (NCT03284645). A total of 98 samples (35 paired intensive CVS and DBS pharmacokinetic samples, 14 paired sparse CVS and DBS samples) from 19 participants were available for this analysis. Results: Swabs were fully saturated within 15 seconds, absorbing 128 µL of plasma matrix with coefficient of variation (%CV) below 1.3%. The method was linear with a weighting factor (1/X) in the range of 25-10000 ng/mL with inter- and intra-day precision (% CV) of 7.69-14.9%, and accuracy (% bias) of 99.1-105.3%. Mean recovery of efavirenz from CVS was 83.8% (%CV, 11.2) with no significant matrix effect. Efavirenz remained stable in swabs for at least 35 days after drying and storage at room temperature. Median (range) CVS efavirenz AUC 0-24h was 16370 ng*h/mL (5803-22088), C max was 1618 ng/mL (610-2438) at a T max of 8.0 h (8.0-12), and C min was 399 ng/mL (110-981). Efavirenz CVS:plasma AUC 0-24h ratio was 0.41 (0.20-0.59). Conclusions: Further application of this method will improve our understanding of the pharmacology of other therapeutics in the female genital tract, including in low- and middle-income countries.
Competing Interests: No competing interests were disclosed.
(Copyright: © 2022 Olagunju A et al.)
Databáze: MEDLINE
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