| Autor: |
Dumas M; The Connecticut Agricultural Experiment Station, Department of Plant Pathology and Ecology, New Haven, CT 06504, U.S.A.; School of Integrative Plant Science, Plant Pathology and Plant-Microbe Biology Section, Cornell University, Ithaca, NY 14850, U.S.A., Borges DF; The Connecticut Agricultural Experiment Station, Department of Plant Pathology and Ecology, New Haven, CT 06504, U.S.A.; Universidade Federal Rural do Semi-Árido, Departamento de Ciências Agronômicas e Florestais, Mossoró 59625-900, RN, Brazil., Priesing S; The Connecticut Agricultural Experiment Station, Department of Plant Pathology and Ecology, New Haven, CT 06504, U.S.A.; School of Integrative Plant Science, Plant Pathology and Plant-Microbe Biology Section, Cornell University, Ithaca, NY 14850, U.S.A.; Biology Department, Southern Connecticut State University, New Haven, CT 06504, U.S.A., Tippett E; The Connecticut Agricultural Experiment Station, Department of Plant Pathology and Ecology, New Haven, CT 06504, U.S.A.; College of Art and Sciences, Ferris State University, Big Rapids, MI 49307, U.S.A.; Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48823, U.S.A., Ambrosio MMQ; Universidade Federal Rural do Semi-Árido, Departamento de Ciências Agronômicas e Florestais, Mossoró 59625-900, RN, Brazil., Luís da Silva W; The Connecticut Agricultural Experiment Station, Department of Plant Pathology and Ecology, New Haven, CT 06504, U.S.A.; Universidade Federal Rural do Semi-Árido, Departamento de Ciências Agronômicas e Florestais, Mossoró 59625-900, RN, Brazil.; College of Agriculture, Health and Natural Resources, University of Connecticut, Storrs, CT 06268, U.S.A. |
| Abstrakt: |
Vineyards in the Southeastern New England American Viticultural Area were surveyed for the incidence of seven major viruses: grapevine leafroll-associated viruses (GLRaV-1, GLRaV-2, GLRaV-3, and GLRaV-4), grapevine fanleaf virus (GFLV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). Viruses were detected by DAS-ELISA and confirmed by RT-PCR and Sanger sequencing. Multiple viruses were present in 19 out of the 25 vineyards surveyed between 2018 and 2020. GLRaV-3 (27.59%) was the most prevalent virus followed by GLRaV-4 (14.90%), GLRaV-1 (13.52%), GLRaV-2 (11.03%), ToRSV (6.34%), GFLV (5.24%), and TRSV (2.62%). Furthermore, phylogenetic analyses of the viral partial genome sequences acquired in this study revealed that the grapevine viruses present in this area are diverse, indicating that they may have been introduced from different sources. Our findings stress the need for improving the sanitary status of planting materials to avoid the introduction and dissemination of viruses to vineyards in this important wine-producing region of New England. |
