Development of a Well-Characterized Cynomolgus Macaque Model of Marburg Virus Disease for Support of Vaccine and Therapy Development.

Autor: Alfson KJ; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Goez-Gazi Y; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Gazi M; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Chou YL; Battelle Biomedical Research Center (BBRC), 1425 Plain City Georgesville Road, West Jefferson, OH 43162, USA., Niemuth NA; Battelle Biomedical Research Center (BBRC), 1425 Plain City Georgesville Road, West Jefferson, OH 43162, USA., Mattix ME; Nonclinical Pathology Services, LLC, 5920 Clubhouse Pointe Dr., Medina, OH 44256, USA., Staples HM; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Klaffke B; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Rodriguez GF; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Bartley C; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Ticer A; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Clemmons EA; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Dutton JW 3rd; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Griffiths A; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA., Meister GT; Battelle Biomedical Research Center (BBRC), 1425 Plain City Georgesville Road, West Jefferson, OH 43162, USA., Sanford DC; Battelle Biomedical Research Center (BBRC), 1425 Plain City Georgesville Road, West Jefferson, OH 43162, USA., Cirimotich CM; Battelle Biomedical Research Center (BBRC), 1425 Plain City Georgesville Road, West Jefferson, OH 43162, USA., Carrion R Jr; Texas Biomedical Research Institute, 8715 W. Military Dr., San Antonio, TX 78227, USA.
Jazyk: angličtina
Zdroj: Vaccines [Vaccines (Basel)] 2022 Aug 14; Vol. 10 (8). Date of Electronic Publication: 2022 Aug 14.
DOI: 10.3390/vaccines10081314
Abstrakt: Marburg virus (MARV) is a filovirus that can infect humans and nonhuman primates (NHPs), causing severe disease and death. Of the filoviruses, Ebola virus (EBOV) has been the primary target for vaccine and therapeutic development. However, MARV has an average case fatality rate of approximately 50%, the infectious dose is low, and there are currently no approved vaccines or therapies targeted at infection with MARV. The purpose of this study was to characterize disease course in cynomolgus macaques intramuscularly exposed to MARV Angola variant. There were several biomarkers that reliably correlated with MARV-induced disease, including: viral load; elevated total clinical scores; temperature changes; elevated ALT, ALP, BA, TBIL, CRP and decreased ALB values; decreased lymphocytes and platelets; and prolonged PTT. A scheduled euthanasia component also provided the opportunity to study the earliest stages of the disease. This study provides evidence for the application of this model to evaluate potential vaccines and therapies against MARV and will be valuable in improving existing models.
Databáze: MEDLINE
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