| Autor: |
Tseilikman V; School of Medical Biology, South Ural State University, 454080 Chelyabinsk, Russia., Akulov A; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Shevelev O; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Khotskina A; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Kontsevaya G; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Moshkin M; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Fedotova J; Laboratory of Neuroendocrinology, Pavlov Institute of Physiology, RAS, 199034 St. Petersburg, Russia., Pashkov A; School of Medical Biology, South Ural State University, 454080 Chelyabinsk, Russia.; FSBI 'Federal Neurosurgical Center', Nemirovich-Danchenko Str. 132/1, 630087 Novosibirsk, Russia., Tseilikman O; School of Medical Biology, South Ural State University, 454080 Chelyabinsk, Russia.; Department of Basic Medicine, Chelyabinsk State University, 454001 Chelyabinsk, Russia., Agletdinov E; AO Vector-Best, Koltsovo Village, Research and Production Zone, Building 36, Room 211, 630559 Novosibirsk, Russia., Tseilikman D; Zelman Institute of Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia., Kondashevskaya M; Avtsyn Research Institute of Human Morphology, 117418 Moscow, Russia., Zavjalov E; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia. |
| Abstrakt: |
A paradoxical reduction in anxiety levels in chronic predator stress paradigm (PS) in Sprague-Dawley rats has recently been shown in previous works. In this paper, we studied the possible neurobiological mechanism of this phenomenon. We segregated PS-exposed Sprague-Dawley rats into the high- and low-anxiety phenotypes. The long-lasting effects of PS on corticosterone levels, blood flow speed in the carotid arteries, diffusion coefficient, and 1H nuclear magnetic resonance spectra in the hippocampus were compared in the high-anxiety and low-anxiety rats. In addition, we evaluated the gene BDNF expression in the hippocampus which is considered to be a main factor of neuroplasticity. We demonstrated that in low-anxiety rats, the corticosterone level was decreased and carotid blood flow speed was increased. Moreover, in the hippocampus of low-anxiety rats compared to the control group and high-anxiety rats, the following changes were observed: (a) a decrease in N-acetyl aspartate levels with a simultaneous increase in phosphoryl ethanol amine levels; (b) an increase in lipid peroxidation levels; (c) a decrease in apparent diffusion coefficient value; (d) an increase in BDNF gene expression. Based on these findings, we proposed that stress-induced anxiety reduction is associated with the elevation of BDNF gene expression directly. Low corticosterone levels and a rise in carotid blood flow speed might facilitate BDNF gene expression. Meanwhile, the decrease in apparent diffusion coefficient value and decrease in N-acetyl aspartate levels, as well as an increase in the lipid peroxidation levels, in the hippocampus possibly reflected destructive changes in the hippocampus. We suggested that in Sprague-Dawley rats, these morphological alterations might be considered as an impetus for further increase in neuroplasticity in the hippocampus. |
