| Autor: |
Riggers DS; Small Animals Department, College of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany., Gurtner C; Institute of Animal Pathology, Department of Infectious Diseases and Pathobiology (DIP), Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland., Protschka M; Institute of Immunology/Molecular Pathogenesis, Center for Biotechnology and Biomedicine, College of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany., Böttcher D; Institute of Veterinary Pathology, College of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany., von Bomhard W; Veterinary Pathology Center, 80689 Munich, Germany., Alber G; Institute of Immunology/Molecular Pathogenesis, Center for Biotechnology and Biomedicine, College of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany., Winter K; Institute of Anatomy, Medical Faculty, Leipzig University, 04103 Leipzig, Germany., Steiner JM; Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA., Heilmann RM; Small Animals Department, College of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany. |
| Abstrakt: |
Diagnosing chronic inflammatory enteropathies (CIE) in cats and differentiation from intestinal lymphoma (IL) using currently available diagnostics is challenging. Intestinally expressed S100/calgranulins, measured in fecal samples, appear to be useful non-invasive biomarkers for canine CIE but have not been evaluated in cats. We hypothesized S100/calgranulins to play a role in the pathogenesis of feline chronic enteropathies (FCE) and to correlate with clinical and/or histologic disease severity. This retrospective case-control study included patient data and gastrointestinal (GI) tissues from 16 cats with CIE, 8 cats with IL, and 16 controls with no clinical signs of GI disease. GI tissue biopsies were immunohistochemically stained using polyclonal α-S100A8/A9 and α-S100A12 antibodies. S100A8/A9 + and S100A12 + cells were detected in all GI segments, with few significant differences between CIE, IL, and controls and no difference between diseased groups. Segmental inflammatory lesions were moderately to strongly correlated with increased S100/calgranulin-positive cell counts. Clinical disease severity correlated with S100A12 + cell counts in cats with IL ( ρ = 0.69, p = 0.042) and more severe diarrhea with colonic lamina propria S100A12 + cells with CIE ( ρ = 0.78, p = 0.021) and duodenal S100A8/A9 + cells with IL ( ρ = 0.71, p = 0.032). These findings suggest a role of the S100/calgranulins in the pathogenesis of the spectrum of FCE, including CIE and IL. |
