| Autor: |
Ibrahim D; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Arisha AH; Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt.; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Khater SI; Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Gad WM; Department of Bacteriology, Animal Health Research Institute (AHRI), Mansoura Branch Agriculture Research Center, Mansoura 35511, Egypt., Hassan Z; Fish Disease Department, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt., Abou-Khadra SH; Microbiology Department, Zagazig Branch, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Zagazig 44511, Egypt., Mohamed DI; Department of Biochemistry, Zagazig Branch, Animal Health Research Institute (AHRI), Agriculture Research Center, Zagazig 44511, Egypt., Ahmed Ismail T; Department of Clinical Laboratory Sciences, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Gad SA; Department of Clinical Pathology, Animal Health Research Institute (AHRI), Zagazig Branch, Agriculture Research Center (ARC), Zagazig 44511, Egypt., Eid SAM; Department of Clinical Pathology, Animal Health Research Institute (AHRI), Zagazig Branch, Agriculture Research Center (ARC), Zagazig 44511, Egypt., Abd El-Wahab RA; Biochemistry department, Animal Health Research Institute (AHRI), Mansoura Branch, Agriculture Research Center (ARC), 246 Dokki, Giza 12618, Egypt., Kishawy ATY; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt. |
| Abstrakt: |
In modern aquaculture, enriching Nile tilapia's diet with omega-3 poly-unsaturated fatty acids (PUFAs) not only plays an important role in its general health but also fortifies its fillet with omega-3-PUFAs. However, the major challenge affecting their delivery is their high instability due to oxidative deterioration. Thus, the prospective incorporation of omega-3-PUFAs into nanocarriers can enhance their stability and bioactivity. In this regard, the effect of reformulated omega-3-NPs was investigated on Nile tilapia's performance, flesh antioxidant stability, immunity, and disease resistance. Four fish groups supplemented with omega-3-PUFAs-loaded nanoparticles (omega-3 NPs) at levels of 0, 1, 2, and 3 g/kg diet and at the end of feeding trial fish challenged with Aeromonas hydrophila . Fish performance (weight gain and feed conversion) was improved in groups supplemented with omega-3-NPs (2 and 3 g/kg diet). The deposition of omega-3-PUFAs in fish flesh elevated with increasing dietary omega-3-NPs. Simultaneously the oxidative markers (H 2 O 2 , MDA, and reactive oxygen species) in fish flesh were reduced, especially with higher omega-3-NPs. Post-challenge, downregulation of IL-1β, IL-6, IL-8, TNF-α, and caspase-1 were noticed after dietary supplementation of omega-3-NPs. Moreover, mRNA expression of autophagy-related genes was upregulated while the mTOR gene was downregulated with higher omega-3 NPs levels. Lower expression of A. hydrophila ahyI and ahyR genes were detected with omega-3 NPs supplementation. In conclusion, omega-3-NPs application can fortify tilapia flesh with omega-3-PUFAs and augment its performance, immunity, and disease resistance against Aeromonas hydrophila . |
