Time and Brain Region-Dependent Excitatory Neurochemical Alterations in Bilateral Common Carotid Artery Occlusion Global Ischemia Model.

Autor: Ojo OB; Biochemical and Molecular Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, 340110, Akure, Nigeria. ojobenedicta@gmail.com.; Sleep Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India. ojobenedicta@gmail.com., Amoo ZA; Biochemical and Molecular Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, 340110, Akure, Nigeria., Olaleye MT; Biochemical and Molecular Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, 340110, Akure, Nigeria., Jha SK; Sleep Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India., Akinmoladun AC; Biochemical and Molecular Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Life Sciences, The Federal University of Technology, PMB 704, 340110, Akure, Nigeria. acakinmoladun@futa.edu.ng.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2023 Jan; Vol. 48 (1), pp. 96-116. Date of Electronic Publication: 2022 Aug 25.
DOI: 10.1007/s11064-022-03732-8
Abstrakt: Strict metabolic regulation in discrete brain regions leads to neurochemical changes in cerebral ischemia. Accumulation of extracellular glutamate is one of the early neurochemical changes that take place during cerebral ischemia. Understanding the sequential neurochemical processes involved in cerebral ischemia-mediated excitotoxicity before the clinical intervention of revascularization and reperfusion may greatly influence future therapeutic strategies for clinical stroke recovery. This study investigated the influence of time and brain regions on excitatory neurochemical indices in the bilateral common carotid artery occlusion (BCCAO) model of global ischemia. Male Wistar rats were subjected to BCCAO for 15 and 60 min to evaluate the effect of ischemia duration on excitatory neurochemical indices (dopamine level, glutamine synthetase, glutaminase, glutamate dehydrogenase, aspartate aminotransferase, monoamine oxidase, acetylcholinesterase, and Na + K + ATPase activities) in the discrete brain regions (cortex, striatum, cerebellum, and hippocampus). BCCAO without reperfusion caused marked time and brain region-dependent alterations in glutamatergic, glutaminergic, dopaminergic, monoaminergic, cholinergic, and electrogenic homeostasis. Prolonged BCCAO decreased cortical, striatal, and cerebellar glutamatergic, glutaminergic, dopaminergic, cholinergic, and electrogenic activities; increased hippocampal glutamatergic, glutaminergic, dopaminergic, and cholinergic activities, increased cortical and striatal monoaminergic activity; decreased cerebellar and hippocampal monoaminergic activity; and decreased hippocampal electrogenic activity. This suggests that excitatory neurotransmitters play a major role in the tissue-specific metabolic plasticity and reprogramming that takes place between the onset of cardiac arrest-mediated global ischemia and clinical intervention of recanalization. These tissue-specific neurochemical indices may serve as diagnostic and therapeutic strategies for mitigating the progression of ischemic damage before revascularization.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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