Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering.

Autor: Ling C; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Peabody GL; Agile BioFoundry, Emeryville, CA, 94608, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA., Salvachúa D; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Kim YM; Agile BioFoundry, Emeryville, CA, 94608, USA.; Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Kneucker CM; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Calvey CH; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA., Monninger MA; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Munoz NM; Agile BioFoundry, Emeryville, CA, 94608, USA.; Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Poirier BC; Agile BioFoundry, Emeryville, CA, 94608, USA.; Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., St John PC; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Woodworth SP; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; Agile BioFoundry, Emeryville, CA, 94608, USA., Magnuson JK; Agile BioFoundry, Emeryville, CA, 94608, USA.; Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Burnum-Johnson KE; Agile BioFoundry, Emeryville, CA, 94608, USA.; Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Guss AM; Agile BioFoundry, Emeryville, CA, 94608, USA. gussam@ornl.gov.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. gussam@ornl.gov., Johnson CW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA. christopher.johnson@nrel.gov.; Agile BioFoundry, Emeryville, CA, 94608, USA. christopher.johnson@nrel.gov., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA. gregg.beckham@nrel.gov.; Agile BioFoundry, Emeryville, CA, 94608, USA. gregg.beckham@nrel.gov.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Aug 22; Vol. 13 (1), pp. 4925. Date of Electronic Publication: 2022 Aug 22.
DOI: 10.1038/s41467-022-32296-y
Abstrakt: Muconic acid is a bioprivileged molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this study, Pseudomonas putida KT2440 is engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hydrolysates, to muconic acid using a model-guided strategy to maximize the theoretical yield. Using adaptive laboratory evolution (ALE) and metabolic engineering in a strain engineered to express the D-xylose isomerase pathway, we demonstrate that mutations in the heterologous D-xylose:H + symporter (XylE), increased expression of a major facilitator superfamily transporter (PP_2569), and overexpression of aroB encoding the native 3-dehydroquinate synthase, enable efficient muconic acid production from glucose and xylose simultaneously. Using the rationally engineered strain, we produce 33.7 g L -1 muconate at 0.18 g L -1 h -1 and a 46% molar yield (92% of the maximum theoretical yield). This engineering strategy is promising for the production of other shikimate pathway-derived compounds from lignocellulosic sugars.
(© 2022. The Author(s).)
Databáze: MEDLINE
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