Experimental infection trials with European North Atlantic ranavirus (Iridoviridae) isolated from lumpfish (Cyclopterus lumpus, L.).

Autor: Scholz F; PHARMAQ Analytiq, Oranmore, Ireland.; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Galway, Ireland., Vendramin N; DTU Aqua, National Institute of Aquatic Resources, Copenhagen, Denmark., Olesen NJ; DTU Aqua, National Institute of Aquatic Resources, Copenhagen, Denmark., Cuenca A; DTU Aqua, National Institute of Aquatic Resources, Copenhagen, Denmark., Moesgaard Iburg T; DTU Aqua, National Institute of Aquatic Resources, Copenhagen, Denmark., Mirimin L; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Galway, Ireland., O'Connor I; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Galway, Ireland., Ruane NM; Marine Institute, Oranmore, Ireland., Rodger HD; VAI Consulting, Kinvara, Ireland., MacCarthy E; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Galway, Ireland.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2022 Nov; Vol. 45 (11), pp. 1745-1756. Date of Electronic Publication: 2022 Aug 21.
DOI: 10.1111/jfd.13696
Abstrakt: European North Atlantic ranavirus (ENARV, Iridoviridae), is a ranavirus species recently isolated from lumpfish (Cyclopterus lumpus, L.), which are used as cleaner fish in Atlantic salmon (Salmo salar) farming in Northern Europe. This study aimed to investigate (1) the virulence of ENARV isolates from Ireland, Iceland and the Faroe Islands to lumpfish; (2) horizontal transmission between lumpfish; and (3) virulence to Atlantic salmon parr. Lumpfish were challenged in a cohabitation model using intraperitoneally (IP) injected shedders, and naïve cohabitants. IP challenge with isolates from Iceland (1.9 × 10 7 TCID 50  ml -1 ) and the Faroe Islands (5.9 × 10 7 TCID 50  ml -1 ) reduced survival in lumpfish, associated with consistent pathological changes. IP challenge with the Irish strain (8.6 × 10 5 TCID 50  ml -1 ) did not significantly reduce survival in lumpfish, but the lower challenge titre complicated interpretation. Horizontal transmission occurred in all strains tested, but no clinical impact was demonstrated in cohabitants. Salmon parr were challenged by IP injection with the Irish isolate, no virulence or virus replication were demonstrated. A ranavirus qPCR assay, previously validated for fish ranaviruses, was first used to detect ENARV in tissues of both in lumpfish and Atlantic salmon. This study provides the first data on the assessment of virulence of ENARV isolates to lumpfish and salmon, guidelines for the diagnosis of ENARV infection, and poses a basis for further investigations into virulence markers.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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