Upregulation of REG IV gene in human intestinal epithelial cells by lipopolysaccharide via downregulation of microRNA-24.

Autor: Takasawa S; Department of Biochemistry, Nara Medical University, Kashihara, Japan., Tsuchida C; Department of Biochemistry, Nara Medical University, Kashihara, Japan., Sakuramoto-Tsuchida S; Department of Biochemistry, Nara Medical University, Kashihara, Japan., Uchiyama T; Department of Biochemistry, Nara Medical University, Kashihara, Japan.; Department of Diagnostic Pathology, Nara Medical University, Kashihara, Japan., Makino M; Department of Biochemistry, Nara Medical University, Kashihara, Japan., Yamauchi A; Department of Biochemistry, Nara Medical University, Kashihara, Japan., Itaya-Hironaka A; Department of Biochemistry, Nara Medical University, Kashihara, Japan.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2022 Sep; Vol. 26 (17), pp. 4710-4720. Date of Electronic Publication: 2022 Aug 09.
DOI: 10.1111/jcmm.17498
Abstrakt: The pathophysiology of inflammatory bowel diseases (IBD) reflects a balance between mucosal injury and reparative mechanisms. Some regenerating gene (Reg) family members (REG Iα, REG Iβ and REG IV) are expressed in Crohn's disease (CD) and ulcerative colitis (UC) and involved as proliferative mucosal factors in IBD. We revealed that REG Iα and REG Iβ were induced in cell culture system by IL-6/IL-22. Although REG IV was upregulated in IBD biopsy samples, the upregulation of REG IV was not at all induced in cell culture by autoimmune-related cytokines such as IL-6, IL-22 and TNFα. Here, we analysed REG IV expression in LS-174 T and HT-29 human intestinal epithelial cells by real-time RT-PCR and elisa. REG IV expression was induced by lipopolysaccharide (LPS). However, LPS did not activate REG IV promoter activity. As the LPS-induced upregulation of REG IV was considered to be regulated post-transcriptionally, we searched targeted microRNA (miR), which revealed that REG IV mRNA has a potential target sequence for miR-24. We measured the miR-24 level of LPS-treated cells and found that the level was significantly lower. The LPS-induced increase of REG IV mRNA was abolished by the introduction of miR-24 mimic but not by non-specific control RNA.
(© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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