The effects of green synthesized anionic cupric oxide nanoparticles on Zaraibi goat spermatozoa during cryopreservation with and without removal of seminal plasma.

Autor: Elshamy AA; Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt., Kotram LE; Immunity Department, Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt., Barakat OS; Agricultural Microbiology Department, Faculty of Agriculture, Cairo University, Giza, Egypt., Mahmoud SM; Biotechnology Department, Faculty of Graduate Studies and Environmental Researches, Ain Shams University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Animal biotechnology [Anim Biotechnol] 2023 Dec; Vol. 34 (7), pp. 2582-2595. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1080/10495398.2022.2106992
Abstrakt: Sperm motility, normal morphology, viability, spermatozoa DNA damage, and lipid peroxidation are all affected by semen cryopreservation. The goal of this study was to see how effective cupric oxide nanoparticles (CuONPs) are as a cryo-extender additive on post-thawed sperm parameters. An artificial vagina was used to collect semen samples from five mature Zaraibi bucks (2-3 years). Ejaculates were pooled and separated into two fractions (A&B), a fraction (A) was left without being centrifuged and a fraction (B) was centrifuged to remove seminal plasma. Both fractions were diluted with tris egg yolk citrate extender (TECE) and then divided into five equal aliquots, each supplemented with (0, 10, 20, 40, and 60 ppm/ml) CuONPs. The findings revealed that removing seminal plasma before cryopreservation harms sperm parameters. Sperm motility, viability index, membrane integrity, biochemical antioxidant marker, DNA integrity, and MDA level improved after supplementation with CuONPs up to 60 ppm/ml, the most prominent significant positive effect was obtained with the highest dose (60 ppm/ml) without removal of the seminal plasm compared to control group. In conclusion: The presence of seminal plasma with a high concentration of CuONPs (up to 60 ppm/ml) may help to mitigate the negative effects of cryo-preservation.
Databáze: MEDLINE
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