Equisetum hyemale-derived unprecedented bioactive composite for hard and soft tissues engineering.

Autor: da Costa E Silva RMF; Universidade Federal da Fronteira Sul, Campus Realeza-PR, Realeza, Paraná, 85770-000, Brazil. rosangela_ferreirafeliz@yahoo.com.br., Diniz IMA; Restorative Dentistry Department, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil., Gomes NA; Restorative Dentistry Department, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil., Silva GJB; Departamento de Engenharia Civil, Universidade Federal de Ouro Preto, Minas Gerais, Ouro Preto, 35400-000, Brazil., da Fonte Ferreira JM; Department of Materials and Ceramic Engineering, CICECO -Aveiro Institute of Materials, University of Aveiro, 3810-193, Aveiro, Portugal., de Freitas Filho RL; Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil., Freitas ETF; Centro de Microscopia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil., Martins DA; Instituto Federal Do Paraná, Umuarama, Paraná, 87507-014, Brazil., Domingues RZ; Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil., Andrade ÂL; Departamento de Química, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Aug 04; Vol. 12 (1), pp. 13425. Date of Electronic Publication: 2022 Aug 04.
DOI: 10.1038/s41598-022-17626-w
Abstrakt: Although Bioactive Glasses (BGs) have been progressively optimized, their preparation often still involves the use of toxic reagents and high calcination temperatures to remove organic solvents. In the present work, these synthesis related drawbacks were overcome by treating the ashes from the Equisetum hyemale plant in an ethanol/water solution to develop a bioactive composite [glass/carbon (BG-Carb)]. The BG-Carb was characterized by scanning electron microscopy, and transmission electron microscopy; and its chemical composition was assessed by inductively coupled plasma-optical emission spectroscopy. Brunauer-Emmett-Teller gas adsorption analysis showed a specific surface area of 121 m 2  g -1 . The formation of hydroxyapatite (HA) surface layer in vitro was confirmed by Fourier-transform infrared spectroscopy analysis before and after immersion in simulated body fluid (SBF) solution. The Rietveld refinement of the XRD patterns and selected area electron diffraction analyses confirmed HA in the sample even before immersing it in SBF solution. However, stronger evidences of the presence of HA were observed after immersion in SBF solution due to the surface mineralization. The BG-Carb samples showed no cytotoxicity on MC3T3-E1 cells and osteo-differentiation capacity similar to the positive control. Altogether, the BG-Carb material data reveals a promising plant waste-based candidate for hard and soft tissue engineering.
(© 2022. The Author(s).)
Databáze: MEDLINE
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