An aco-2::gfp knock-in enables the monitoring of mitochondrial morphology throughout C. elegans lifespan.

Autor:	Begelman DV; The Buck Institute for Research on Aging., Woods G; The Buck Institute for Research on Aging., Bhaumik D; The Buck Institute for Research on Aging., Angeli S; University of Maine, Molecular & Biomedical Sciences.; The Buck Institute for Research on Aging., Foulger AC; The Buck Institute for Research on Aging., Lucanic M; GeroStateAlpha.; The Buck Institute for Research on Aging., Lan J; Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, the Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, China., Andersen JK; The Buck Institute for Research on Aging., Lithgow GJ; The Buck Institute for Research on Aging.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2022 Jul 17; Vol. 2022. Date of Electronic Publication: 2022 Jul 17 (Print Publication: 2022).
DOI:	10.17912/micropub.biology.000599
Abstrakt:	We used CRISPR/Cas9 gene editing in C. elegans in order to fluorescently tag endogenous aconitase-2 (ACO-2). ACO-2 is a mitochondrially localized protein, and the aco-2::gfp strain enabled the examination of native mitochondrial morphology in live animals. Here we validate that the aco-2::gfp strain displays the prototypic changes in mitochondrial morphology known to occur during aging and upon paraquat (PQ) induced mitochondrial stress. We also provide evidence that the ACO-2::GFP reporter can serve as a superior means for tracking mitochondrial morphology than conventional MitoTracker dyes-especially in aged-worms. (Copyright: © 2022 by the authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu