Estimating genotypic richness and proportion of identical multi-locus genotypes in aquatic microalgal populations.

Autor:	Sassenhagen I; Department of Ecology and Genetics/Limnology, Uppsala University, Uppsala, Sweden., Erdner DL; Marine Science Institute, The University of Texas at Austin, Port Aransas, TX, USA., Lougheed BC; Department of Earth Sciences, Uppsala University, Uppsala, Sweden., Richlen ML; Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA., SjÖqvist C; Environmental and Marine Biology, Åbo Akademi University, Turku, Finland.
Jazyk:	angličtina
Zdroj:	Journal of plankton research [J Plankton Res] 2022 Jul 16; Vol. 44 (4), pp. 559-572. Date of Electronic Publication: 2022 Jul 16 (Print Publication: 2022).
DOI:	10.1093/plankt/fbac034
Abstrakt:	The majority of microalgal species reproduce asexually, yet population genetic studies rarely find identical multi-locus genotypes (MLG) in microalgal blooms. Instead, population genetic studies identify large genotypic diversity in most microalgal species. This paradox of frequent asexual reproduction but low number of identical genotypes hampers interpretations of microalgal genotypic diversity. We present a computer model for estimating, for the first time, the number of distinct MLGs by simulating microalgal population composition after defined exponential growth periods. The simulations highlighted the effects of initial genotypic diversity, sample size and intraspecific differences in growth rates on the probability of isolating identical genotypes. We estimated the genotypic richness for five natural microalgal species with available high-resolution population genetic data and monitoring-based growth rates, indicating 500 000 to 2 000 000 distinct genotypes for species with few observed clonal replicates (<5%). Furthermore, our simulations indicated high variability in genotypic richness over time and among microalgal species. Genotypic richness was also strongly impacted by intraspecific variability in growth rates. The probability of finding identical MLGs and sampling a representative fraction of genotypes decreased noticeably with smaller sample sizes, challenging the detection of differences in genotypic diversity with typical isolate numbers in the field. (© The Author(s) 2022. Published by Oxford University Press.)
Databáze:	MEDLINE
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