| Autor: |
Stasyuk N; Institute of Cell Biology National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.; Department of Biology and Chemistry, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine., Demkiv O; Institute of Cell Biology National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine., Gayda G; Institute of Cell Biology National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine., Zakalskiy A; Institute of Cell Biology National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.; Institute of Animal Biology of the National Academy of Agrarian Sciences of Ukraine, 79034 Lviv, Ukraine., Klepach H; Department of Biology and Chemistry, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine., Bisko N; M. G. Kholodny Botany Institute, National Academy of Sciences of Ukraine, 01601 Kyiv, Ukraine., Gonchar M; Institute of Cell Biology National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.; Department of Biology and Chemistry, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine., Nisnevitch M; Department of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, Israel. |
| Abstrakt: |
Metallic nanoparticles potentially have wide practical applications in various fields of science and industry. In biosensorics, they usually act as catalysts or nanozymes (NZs) and as mediators of electron transfer. We describe here the development of amperometric biosensors (ABSs) based on purified oxidases, synthesized nanoparticles of CuCe (nCuCe), and micro/nanoporous gold (pAu), which were electro-deposited on a graphite electrode (GE). As an effective peroxidase (PO)-like NZ, nCuCe was used here as a hydrogen-peroxide-sensing platform in ABSs that were based on glucose oxidase, alcohol oxidase, methylamine oxidase, and L-arginine oxidase. At the same time, nCuCe is an electroactive mediator and has been used in laccase-based ABSs. As a result, the ABSs we constructed and characterized were based on glucose, methanol, methyl amine, L-arginine, and catechol, respectively. The developed nCuCe-based ABSs exhibited improved analytical characteristics in comparison with the corresponding PO-based ABSs. Additionally, the presence of pAu, with its extremely advanced chemo-sensing surface layer, was shown to significantly increase the sensitivities of all constructed ABSs. As an example, the bioelectrodes containing laccase/GE, laccase/nCuCe/GE, and laccase/nCuCe/pAu/GE exhibited sensitivities to catechol at 2300, 5055, and 9280 A·M -1 ·m -2 , respectively. We demonstrate here that pAu is an effective carrier of electroactive nanomaterials coupled with oxidases, which may be promising in biosensors. |
