Root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism.

Autor: Fusi R; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Future Food Beacon of Excellence, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Rosignoli S; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Lou H; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Urrbrae, SA 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Glen Osmond, SA 5062, Australia., Sangiorgi G; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Bovina R; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Pattem JK; Food Sciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; National Centre for Molecular Hydrodynamics, and Soft Matter Biomaterials and Bio-interfaces, University of Nottingham, LE12 5RD, United Kingdom., Borkar AN; School of Veterinary Medicine and Science, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Wolfson Center for Global Virus Research, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Lombardi M; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Department of Science and Technology for Humans and the Environment, Università Campus Bio-Medico di Roma, Rome, 00128 Italy., Forestan C; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Milner SG; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Davis JL; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Lale A; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Future Food Beacon of Excellence, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Kirschner GK; Institute of Crop Sciences and Resource Conservation, Crop Functional Genomics, University of Bonn, 53113 Bonn, Germany., Swarup R; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Tassinari A; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Pandey BK; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Future Food Beacon of Excellence, University of Nottingham, LE12 5RD Nottingham, United Kingdom., York LM; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830., Atkinson BS; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Sturrock CJ; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Mooney SJ; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Hochholdinger F; Institute of Crop Sciences and Resource Conservation, Crop Functional Genomics, University of Bonn, 53113 Bonn, Germany., Tucker MR; School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Urrbrae, SA 5064, Australia.; Australian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Glen Osmond, SA 5062, Australia., Himmelbach A; Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gaterslelben, Germany., Stein N; Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gaterslelben, Germany.; Center of integrated Breeding Research, Department of Crop Sciences, Georg-August-University, 37075 Göttingen, Germany., Mascher M; Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gaterslelben, Germany.; German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig, 04103 Leipzig, Germany., Nagel KA; Institute of Bio- and Geo-sciences, Plant Sciences, 52428 Juelich, Germany., De Gara L; Department of Science and Technology for Humans and the Environment, Università Campus Bio-Medico di Roma, Rome, 00128 Italy., Simmonds J; John Innes Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom., Uauy C; John Innes Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom., Tuberosa R; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy., Lynch JP; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Department of Plant Science, Pennsylvania State University, University Park, PA 16802., Yakubov GE; Food Sciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Bennett MJ; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Future Food Beacon of Excellence, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Bhosale R; School of Biosciences, University of Nottingham, LE12 5RD Nottingham, United Kingdom.; Future Food Beacon of Excellence, University of Nottingham, LE12 5RD Nottingham, United Kingdom., Salvi S; Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Aug 02; Vol. 119 (31), pp. e2201350119. Date of Electronic Publication: 2022 Jul 26.
DOI: 10.1073/pnas.2201350119
Abstrakt: Root angle in crops represents a key trait for efficient capture of soil resources. Root angle is determined by competing gravitropic versus antigravitropic offset (AGO) mechanisms. Here we report a root angle regulatory gene termed ENHANCED GRAVITROPISM1 ( EGT1 ) that encodes a putative AGO component, whose loss-of-function enhances root gravitropism. Mutations in barley and wheat EGT1 genes confer a striking root phenotype, where every root class adopts a steeper growth angle. EGT1 encodes an F-box and Tubby domain-containing protein that is highly conserved across plant species. Haplotype analysis found that natural allelic variation at the barley EGT1 locus impacts root angle. Gravitropic assays indicated that Hvegt1 roots bend more rapidly than wild-type. Transcript profiling revealed Hvegt1 roots deregulate reactive oxygen species (ROS) homeostasis and cell wall-loosening enzymes and cofactors. ROS imaging shows that Hvegt1 root basal meristem and elongation zone tissues have reduced levels. Atomic force microscopy measurements detected elongating Hvegt1 root cortical cell walls are significantly less stiff than wild-type. In situ analysis identified HvEGT1 is expressed in elongating cortical and stele tissues, which are distinct from known root gravitropic perception and response tissues in the columella and epidermis, respectively. We propose that EGT1 controls root angle by regulating cell wall stiffness in elongating root cortical tissue, counteracting the gravitropic machinery's known ability to bend the root via its outermost tissues. We conclude that root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism.
Databáze: MEDLINE
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