Whole Blood Dendritic Cell Cytokine Production Assay.

Autor:	Woodberry T; Menzies School of Health Research and Charles Darwin University, Darwin, NT, Australia., Loughland JR; Menzies School of Health Research and Charles Darwin University, Darwin, NT, Australia.; QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia., Minigo G; Menzies School of Health Research and Charles Darwin University, Darwin, NT, Australia. gabriela.minigo@cdu.edu.au.; College of Health and Human Sciences, Charles Darwin University, Darwin, NT, Australia. gabriela.minigo@cdu.edu.au.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2470, pp. 715-729.
DOI:	10.1007/978-1-0716-2189-9_53
Abstrakt:	This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c + DC, CD141 + DC, and CD16 + DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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