Genetic modification of flavone biosynthesis in rice enhances biofilm formation of soil diazotrophic bacteria and biological nitrogen fixation.

Autor: Yan D; Department of Plant Sciences, University of California, Davis, California, USA., Tajima H; Department of Plant Sciences, University of California, Davis, California, USA., Cline LC; Bayer Crop Science, St. Louis, Missouri, USA., Fong RY; Department of Nutrition, University of California, Davis, California, USA., Ottaviani JI; Department of Nutrition, University of California, Davis, California, USA.; Mars Inc., McLean, Virginia, USA., Shapiro HY; Department of Plant Sciences, University of California, Davis, California, USA., Blumwald E; Department of Plant Sciences, University of California, Davis, California, USA.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2022 Nov; Vol. 20 (11), pp. 2135-2148. Date of Electronic Publication: 2022 Aug 08.
DOI: 10.1111/pbi.13894
Abstrakt: Improving biological nitrogen fixation (BNF) in cereal crops is a long-sought objective; however, no successful modification of cereal crops showing increased BNF has been reported. Here, we described a novel approach in which rice plants were modified to increase the production of compounds that stimulated biofilm formation in soil diazotrophic bacteria, promoted bacterial colonization of plant tissues and improved BNF with increased grain yield at limiting soil nitrogen contents. We first used a chemical screening to identify plant-produced compounds that induced biofilm formation in nitrogen-fixing bacteria and demonstrated that apigenin and other flavones induced BNF. We then used CRISPR-based gene editing targeting apigenin breakdown in rice, increasing plant apigenin contents and apigenin root exudation. When grown at limiting soil nitrogen conditions, modified rice plants displayed increased grain yield. Biofilm production also modified the root microbiome structure, favouring the enrichment of diazotrophic bacteria recruitment. Our results support the manipulation of the flavone biosynthetic pathway as a feasible strategy for the induction of biological nitrogen fixation in cereals and a reduction in the use of inorganic nitrogen fertilizers.
(© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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