Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation.

Autor: Mendes Soares IP; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Anselmi C; Department of Genetics, Morphology, Orthodontics, and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Guiné I; Department of Genetics, Morphology, Orthodontics, and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Fernandes LO; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Pires MLBA; Department of Genetics, Morphology, Orthodontics, and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., de Souza Costa CA; Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Scheffel DLS; Department of Dentistry, Maringá State University (UEM), Maringá, Paraná, Brazil., Hebling J; Department of Genetics, Morphology, Orthodontics, and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil. Electronic address: josimeri.hebling@unesp.br.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2022 Sep; Vol. 124, pp. 104237. Date of Electronic Publication: 2022 Jul 18.
DOI: 10.1016/j.jdent.2022.104237
Abstrakt: Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation.
Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX - inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%).
Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control.
Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days.
Clinical Significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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