| Autor: |
Wheeler LR; College of Veterinary Medicine, Texas A&M University, College Station, TX, USA., Edwards TH; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA.; BluePearl Veterinary Specialists and the College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA., Heinz JA; College of Veterinary Medicine, Texas A&M University, College Station, TX, USA., Scott LLF; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA., Grantham LE 2nd; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA.; Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA., Keesee JD; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA., Henderson AF; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA., Gerardo AC; Department of Defense Military Working Dog Veterinary Service, JBSA Lackland Air Force Base, TX, USA., Hoareau G; Department of Surgery, University of Utah Health, Salt Lake City, UT, USA., Bynum JA; U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, USA. |
| Abstrakt: |
The TEG 6s (Haemonetics) point-of-care viscoelastic analyzer is portable, compact, simple to use, and has the potential for rapid viscoelastic analysis that can guide the treatment of veterinary patients at the site of care. Although approved for use in people, the TEG 6s has yet to be evaluated for hemostatic analysis in veterinary medicine. Citrated whole blood (CWB) was collected from 27 healthy dogs. An aliquot of CWB from each dog was diluted by 33% with an isotonic crystalloid, representing an in vitro model of hemodilution. Unaltered and diluted CWB samples were analyzed using 2 TEG 6s and 6 TEG 5000 (Haemonetics) analyzers. The 6 TEG 5000 analyzers ran duplicate analyses of either unaltered or diluted samples using 1 of 3 reagents (Haemonetics): Kaolin TEG, RapidTEG, or TEG Functional Fibrinogen. Duplicate TEG 5000 analyses were averaged and compared with a single TEG 6s analysis. Lin concordance correlation coefficient and Bland-Altman plots were used to evaluate agreement of reaction time, kinetic time, alpha angle, maximum amplitude (MA), and G value (G) for samples activated with Kaolin TEG, and agreement of MA for samples activated with RapidTEG between the 2 machines. Overall, agreement between the TEG 6s and TEG 5000 analyzers was poor. Viscoelastic measurements by the TEG 6s and TEG 5000 in healthy dogs were not all interchangeable. Agreement was satisfactory only for MA and G measurements of diluted blood samples activated with Kaolin TEG, and MA measurements for both unaltered and diluted blood samples activated with RapidTEG. |
