A collagen plug with shape memory to seal iatrogenic fetal membrane defects after fetoscopic surgery.

Autor:	Meuwese RTC; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Versteeg EMM; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., van Drongelen J; Department of Obstetrics & Gynecology, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., de Hoog D; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Bouwhuis D; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Vandenbussche FPHA; Department of Obstetrics & Gynecology, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands.; Department of Obstetrics & Gynecology, Helios Klinikum Duisburg, An der Abtei 7, 47166, Duisburg, Germany., van Kuppevelt TH; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands., Daamen WF; Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands.
Jazyk:	angličtina
Zdroj:	Bioactive materials [Bioact Mater] 2022 Jun 24; Vol. 20, pp. 463-471. Date of Electronic Publication: 2022 Jun 24 (Print Publication: 2023).
DOI:	10.1016/j.bioactmat.2022.06.007
Abstrakt:	Iatrogenic preterm premature rupture of fetal membranes (iPPROM) after fetal surgery remains a strong trigger for premature birth. As fetal membrane defects do not heal spontaneously and amniotic fluid leakage and chorioamniotic membrane separation may occur, we developed a biocompatible, fetoscopically-applicable collagen plug with shape memory to prevent leakage. This plug expands directly upon employment and seals fetal membranes, hence preventing amniotic fluid leakage and potentially iPPROM. Lyophilized type I collagen plugs were given shape memory and crimped to fit through a fetoscopic cannula (Ø 3 mm). Expansion of the plug was examined in phosphate buffered saline (PBS). Its sealing capacity was studied ex vivo using human fetal membranes, and in situ in a porcine bladder model. The crimped plug with shape memory expanded and tripled in diameter within 1 min when placed into PBS, whereas a crimped plug without shape memory did not. In both human fetal membranes and porcine bladder, the plug expanded in the defect, secured itself and sealed the defect without membrane rupture. In conclusion, collagen plugs with shape memory are promising as medical device for rapid sealing of fetoscopic defects in fetal membranes at the endoscopic entry point. Competing Interests: WD, RM, and TvK are mentioned as inventors on related patent applications. Other authors: no conflicts of interest. (© 2022 The Authors.)
Databáze:	MEDLINE
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