Genome-Wide Characterization of Superoxide Dismutase (SOD) Genes in Daucus carota : Novel Insights Into Structure, Expression, and Binding Interaction With Hydrogen Peroxide (H 2 O 2 ) Under Abiotic Stress Condition.
| Autor: | Zameer R; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan., Fatima K; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan., Azeem F; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan., ALgwaiz HIM; Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia., Sadaqat M; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan., Rasheed A; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan., Batool R; Department of Botany, GC Women University, Faisalabad, Pakistan., Shah AN; Department of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan., Zaynab M; Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China., Shah AA; Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan., Attia KA; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia., AlKahtani MDF; Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia., Fiaz S; Department of Plant Breeding and Genetics, The University of Haripur, Haripur, Pakistan. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in plant science [Front Plant Sci] 2022 Jun 08; Vol. 13, pp. 870241. Date of Electronic Publication: 2022 Jun 08 (Print Publication: 2022). |
| DOI: | 10.3389/fpls.2022.870241 |
| Abstrakt: | Superoxide dismutase (SOD) proteins are important antioxidant enzymes that help plants to grow, develop, and respond to a variety of abiotic stressors. SOD gene family has been identified in a number of plant species but not yet in Daucus carota . A total of 9 DcSOD genes, comprising 2 FeSODs, 2 MnSODs, and 5 Cu/ZnSODs, are identified in the complete genome of D. carota , which are dispersed in five out of nine chromosomes. Based on phylogenetic analysis, SOD proteins from D. carota were categorized into two main classes (Cu/ZnSODs and MnFeSODs). It was predicted that members of the same subgroups have the same subcellular location. The phylogenetic analysis was further validated by sequence motifs, exon-intron structure, and 3D protein structures, with each subgroup having a similar gene and protein structure. Cis -regulatory elements responsive to abiotic stresses were identified in the promoter region, which may contribute to their differential expression. Based on RNA-seq data, tissue-specific expression revealed that DcCSD2 had higher expression in both xylem and phloem. Moreover, DcCSD2 was differentially expressed in dark stress. All SOD genes were subjected to qPCR analysis after cold, heat, salt, or drought stress imposition. SODs are antioxidants and play a critical role in removing reactive oxygen species (ROS), including hydrogen peroxide (H Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Zameer, Fatima, Azeem, ALgwaiz, Sadaqat, Rasheed, Batool, Shah, Zaynab, Shah, Attia, AlKahtani and Fiaz.) |
| Databáze: | MEDLINE |
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