Desorption Electrospray Ionization Mass Spectrometry Imaging Illustrates the Quality Characters of Isatidis Radix.

Autor:	Nie LX; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China.; WHO Collaborating Center for Herbal Medicine (CHN-139), Beijing, China., Huang LY; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China., Wang XP; Hebei University of Chinese Medicine, Shijiazhuang, China., Lv LF; Shaoxing Institutes for Food and Drug Control, Shaoxing, China., Yang XX; Waters Corporation, Beijing, China., Jia XF; Waters Corporation, Beijing, China., Kang S; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China., Yao LW; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China., Dai Z; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China., Ma SC; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, China.; WHO Collaborating Center for Herbal Medicine (CHN-139), Beijing, China.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Jun 15; Vol. 13, pp. 897528. Date of Electronic Publication: 2022 Jun 15 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.897528
Abstrakt:	For a long history, herbal medicines have made significant contributions to human health all around the world. However, the exploration of an effective approach to illustrate their inner quality remains a challenge. So, it is imperative to develop new methods and technologies to characterize and identify quality markers of herbal medicines. Taking Isatidis Radix, the dried root of Isatis indigotica as an example, desorption electrospray ionization (DESI), in combination with quadrupole-time-of-flight mass spectrometry (Q-TOF/MS), was applied in this work for the first time to reveal the comprehensive spatial distribution of metabolites and, further, to illustrate quality characters of this herbal medicine. After simple pretreatment, 102 metabolites including alkaloids, sulfur-containing compounds, phenylpropanoids, nucleosides, amino acids, organic acids, flavonoids, phenols, terpenes, saccharides, peptides, and sphingolipids were characterized, some of which were successfully localized and visualized in the transverse section of the root. Based on the ion images, samples with different quality characters were distinguished unambiguously by the pattern recognition method of orthogonal partial least squares discrimination analysis (OPLS-DA). Simultaneously, 11 major influencing components exerting higher ion intensities in superior samples were identified as the potential quality markers of Isatidis Radix. Desorption electrospray ionization (DESI) mass spectrometry imaging (MSI), together with chemometric analysis could not only improve the understanding of the plant biology of herbal medicines but also be beneficial in the identification of quality markers, so as to carry out better quality control of herbal medicines. Competing Interests: X-XY and X-FJ are employed by Waters Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Nie, Huang, Wang, Lv, Yang, Jia, Kang, Yao, Dai and Ma.)
Databáze:	MEDLINE
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