Curcumin Plant for Colorectal Cancer Prediction and Prevention Using In Silico Molecular Analysis; HOT-MELT Extrusion.

Autor: Muthu Mohamed JM; College of Pharmacy, Shri Indra Ganesan Institute of Medical Science, Manikandam, Tiruchirapalli 620012, Tamil Nadu, India., Kavitha K; Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, India., Ahmad F; Department of Anesthesia Technology, College of Applied Medical Sciences, Jubail, Imam Abdulrahman Bin Faisal University, P.O. Box 4030, Jubail, Saudi Arabia., Sherbiny ME; Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 1666, Riyadh 11597, Saudi Arabia., Ebrahim D; Department of Respiratory Care, College of Applied Medical Sciences, Jubail, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., El-Sagheer AM; Department of Neuroscience, College of Applied Medical Sciences, Jubail, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Ebrahim HA; Department of Basic Medical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia., Abdelmonem Elsherbini DM; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia.; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Ebrahim Abdelrahman MA; Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 1666, Riyadh 11597, Saudi Arabia., Dejene M; Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2022 Jun 23; Vol. 2022, pp. 4376960. Date of Electronic Publication: 2022 Jun 23 (Print Publication: 2022).
DOI: 10.1155/2022/4376960
Abstrakt: The impact of a soluble complex (SC) of curcumin (CuR) synthesized using hot melt (HM) and hot-melt extrusion (HE) technologies on adenocarcinoma cells for the treatment of colorectal cancer by enhancing CuR solubility is investigated in this work. In silico molecular modelling, solubility, drug release, and physicochemical analysis were all part of the phase solubility (PS) study, which featured a novel dyeing test and a central composite design to optimize the best complex (CDD). The optimal HE-SC (1 : 5) enhances solubility (0.8521 ± 0.016 mg·mL -1 ) and dissolution (91.87 ± 0.208% at 30 min), and it has an ideal stability constant (309 and 377 M -1 ) at 25 and 37°C and an A L type of isotherm, implying 1 : 1 stoichiometry according to the findings. An intermolecular hydrogen bond that has not undergone any chemical change and has resulted in the complete conversion of the amorphous form aids in the creation of SC. In vitro cytotoxicity was measured at IC 50 on the SW480 (72 M·mL -1 ) and Caco-2 (40 M·mL -1 ) cells. According to apoptotic studies, apoptosis was responsible for the vast majority of cell death, with necrosis accounting for a small proportion of the total. In vivo toxicity was established using a zebrafish model, and a western blot examination revealed apoptosis at the molecular level. It was argued that the novel formulations developed using HE technology are more significant and effective than existing pure CuR formulations.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2022 Jamal Moideen Muthu Mohamed et al.)
Databáze: MEDLINE
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