Tissue-engineered composite tracheal grafts create mechanically stable and biocompatible airway replacements.

Autor: Liu L; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA., Dharmadhikari S; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH, USA., Spector BM; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA., Tan ZH; College of Medicine, The Ohio State University, Columbus, OH, USA., Van Curen CE; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA., Agarwal R; College of Medicine, The Ohio State University, Columbus, OH, USA., Nyirjesy S; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA.; College of Medicine, The Ohio State University, Columbus, OH, USA., Shontz K; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA., Sperber SA; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA., Breuer CK; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.; Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH, USA., Zhao K; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA., Reynolds SD; Center for Perinatal Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA., Manning A; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH, USA.; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA.; College of Medicine, The Ohio State University, Columbus, OH, USA., VanKoevering KK; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA.; College of Medicine, The Ohio State University, Columbus, OH, USA., Chiang T; Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, OH, USA.; Department of Otolaryngology-Head & Neck Surgery, The Ohio State University Medical Center, Columbus, OH, USA.; College of Medicine, The Ohio State University, Columbus, OH, USA.
Jazyk: angličtina
Zdroj: Journal of tissue engineering [J Tissue Eng] 2022 Jun 26; Vol. 13, pp. 20417314221108791. Date of Electronic Publication: 2022 Jun 26 (Print Publication: 2022).
DOI: 10.1177/20417314221108791
Abstrakt: We tested composite tracheal grafts (CTG) composed of a partially decellularized tracheal graft (PDTG) combined with a 3-dimensional (3D)-printed airway splint for use in long-segment airway reconstruction. CTG is designed to recapitulate the 3D extracellular matrix of the trachea with stable mechanical properties imparted from the extraluminal airway splint. We performed segmental orthotopic tracheal replacement in a mouse microsurgical model. MicroCT was used to measure graft patency. Tracheal neotissue formation was quantified histologically. Airflow dynamic properties were analyzed using computational fluid dynamics. We found that CTG are easily implanted and did not result in vascular erosion, tracheal injury, or inflammation. Graft epithelialization and endothelialization were comparable with CTG to control. Tracheal collapse was absent with CTG. Composite tracheal scaffolds combine biocompatible synthetic support with PDTG, supporting the regeneration of host epithelium while maintaining graft structure.
Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(© The Author(s) 2022.)
Databáze: MEDLINE
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