Intestinal Microbial Composition of Children in a Randomized Controlled Trial of Probiotics to Treat Acute Gastroenteritis.

Autor:	Horne RG; Cell Biology Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada., Freedman SB; Sections of Pediatric Emergency Medicine and Gastroenterology, Department of Pediatrics, Alberta Children's Hospital, Alberta Children's Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Johnson-Henry KC; Cell Biology Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada., Pang XL; Alberta Precision Laboratories - Public Health Laboratory (ProvLab), Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada., Lee BE; Women and Children's Research Institute, Stollery Children's Hospital, University of Alberta, Edmonton, AB, Canada., Farion KJ; Children's Hospital of Eastern Ontario, University of Ottawa, Ottawa, ON, Canada., Gouin S; Departments of Emergency Medicine and Pediatrics, Centre Hospitalier Universitaire (CHU) Sainte-Justine, Université de Montréal, Montréal, QC, Canada., Schuh S; Division of Emergency Medicine, Department of Paediatrics, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada., Poonai N; Division of Pediatric Emergency Medicine, London Children's Hospital Health Science Centre, Department of Pediatrics, Western University, London, ON, Canada., Hurley KF; Pediatric Emergency Medicine, Izaak Walton Killam (IWK) Children's Hospital, Dalhousie University, Halifax, NS, Canada., Finkelstein Y; Division of Emergency Medicine, Department of Paediatrics, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada., Xie J; Pediatric Emergency Medicine, Izaak Walton Killam (IWK) Children's Hospital, Dalhousie University, Halifax, NS, Canada., Williamson-Urquhart S; Section of Pediatric Emergency Medicine, Department of Pediatrics, Alberta Children's Hospital, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Chui L; Alberta Precision Laboratories - Public Health Laboratory (ProvLab), Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada., Rossi L; Department of Biochemistry and Biomedical Sciences, McMaster University Medical Centre, Hamilton, ON, Canada., Surette MG; Department of Biochemistry and Biomedical Sciences, McMaster University Medical Centre, Hamilton, ON, Canada., Sherman PM; Cell Biology Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada.; Division of Gastroenterology, Hepatology and Nutrition, Department of Paediatrics, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jun 14; Vol. 12, pp. 883163. Date of Electronic Publication: 2022 Jun 14 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.883163
Abstrakt:	Compositional analysis of the intestinal microbiome in pre-schoolers is understudied. Effects of probiotics on the gut microbiota were evaluated in children under 4-years-old presenting to an emergency department with acute gastroenteritis. Included were 70 study participants (n=32 placebo, n=38 probiotics) with stool specimens at baseline (day 0), day 5, and after a washout period (day 28). Microbiota composition and deduced functions were profiled using 16S ribosomal RNA sequencing and predictive metagenomics, respectively. Probiotics were detected at day 5 of administration but otherwise had no discernable effects, whereas detection of bacterial infection (P<0.001) and participant age (P<0.001) had the largest effects on microbiota composition, microbial diversity, and deduced bacterial functions. Participants under 1 year had lower bacterial diversity than older aged pre-schoolers; compositional changes of individual bacterial taxa were associated with maturation of the gut microbiota. Advances in age were associated with differences in gut microbiota composition and deduced microbial functions, which have the potential to impact health later in life. Clinical Trial Registration: www.ClinicalTrials.gov, identifier: NCT01853124. Competing Interests: SF is supported by the Alberta Children’s Hospital Professorship in Child Health and Wellness. MS is the recipient of a Canadian Research Chair in Interdisciplinary Microbiome Research. YF is the recipient of the Canada Research Chair in Pediatric Drug Safety and Efficacy. PS is the recipient of a Canadian Research Chair in Gastrointestinal Disease and research funded by the Canadian Institutes of Health Research (MOP-89894 and IOP-92890) and received honoraria from Abbott Nutrition, Mead Johnson Nutritionals and Nestlé Nutrition. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Horne, Freedman, Johnson-Henry, Pang, Lee, Farion, Gouin, Schuh, Poonai, Hurley, Finkelstein, Xie, Williamson-Urquhart, Chui, Rossi, Surette and Sherman.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu