An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea.

Autor: Buessecker S; Department of Earth System Science, Stanford University, Stanford, CA, USA. sbuessecker@stanford.edu., Palmer M; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA. marike.palmer@unlv.edu., Lai D; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Dimapilis J; Department of Biology, California State University, San Bernardino, CA, USA., Mayali X; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Mosier D; Department of Biology, California State University, San Bernardino, CA, USA.; Department of Geoscience, University of Calgary, Calgary, AB, Canada., Jiao JY; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China., Colman DR; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Keller LM; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., St John E; Department of Biology, Portland State University, Portland, OR, USA., Miranda M; Department of Biology, California State University, San Bernardino, CA, USA., Gonzalez C; Department of Biology, California State University, San Bernardino, CA, USA., Gonzalez L; Department of Biology, California State University, San Bernardino, CA, USA., Sam C; Department of Biology, California State University, San Bernardino, CA, USA., Villa C; Department of Biology, California State University, San Bernardino, CA, USA., Zhuo M; Department of Biology, California State University, San Bernardino, CA, USA., Bodman N; Department of Biology, California State University, San Bernardino, CA, USA., Robles F; Department of Biology, California State University, San Bernardino, CA, USA., Boyd ES; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Cox AD; Department of Chemistry and Geochemistry, Montana Technological University, Butte, MT, USA., St Clair B; Department of Chemistry and Geochemistry, Montana Technological University, Butte, MT, USA., Hua ZS; Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, PR China., Li WJ; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.; College of Fisheries, Henan Normal University, Xinxiang, PR China., Reysenbach AL; Department of Biology, Portland State University, Portland, OR, USA., Stott MB; School of Biological Sciences, University of Canterbury, Christchurch, New Zealand., Weber PK; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Pett-Ridge J; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.; Life & Environmental Sciences Department, University of Merced, Merced, CA, USA., Dekas AE; Department of Earth System Science, Stanford University, Stanford, CA, USA., Hedlund BP; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Dodsworth JA; Department of Biology, California State University, San Bernardino, CA, USA. JDodsworth@csusb.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jun 30; Vol. 13 (1), pp. 3773. Date of Electronic Publication: 2022 Jun 30.
DOI: 10.1038/s41467-022-31452-8
Abstrakt: Trace metals have been an important ingredient for life throughout Earth's history. Here, we describe the genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota), Wolframiiraptor gerlachensis, and its growth dependence on tungsten. A metagenome-assembled genome (MAG) of W. gerlachensis encodes putative tungsten membrane transport systems, as well as pathways for anaerobic oxidation of sugars probably mediated by tungsten-dependent ferredoxin oxidoreductases that are expressed during growth. Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. Phylogenetic analyses of 78 high-quality Wolframiiraptoraceae MAGs from terrestrial and marine hydrothermal systems suggest that tungsten-associated enzymes were present in the last common ancestor of extant Wolframiiraptoraceae. Our observations imply a crucial role for tungsten-dependent metabolism in the origin and evolution of this lineage, and hint at a relic metabolic dependence on this trace metal in early anaerobic thermophiles.
(© 2022. The Author(s).)
Databáze: MEDLINE
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