Severe Acute Respiratory Syndrome Coronavirus 2 Is Detected in the Gastrointestinal Tract of Asymptomatic Endoscopy Patients but Is Unlikely to Pose a Significant Risk to Healthcare Personnel.

Autor:	Cherne MD; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Gentry AB; Department of Gastroenterology, Bozeman Health Deaconess Hospital, Bozeman, Montana., Nemudraia A; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Nemudryi A; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Hedges JF; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Walk H; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Blackwell K; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Snyder DT; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Jerome M; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Madden W; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana.; Rollins School of Public Heath, Emory University, Atlanta, Georgia., Hashimi M; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Sebrell TA; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., King DB; Department of Clinical Research, Bozeman Health Deaconess Hospital, Bozeman, Montana., Plowright RK; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Jutila MA; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Wiedenheft B; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana., Bimczok D; Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana.
Jazyk:	angličtina
Zdroj:	Gastro hep advances [Gastro Hep Adv] 2022; Vol. 1 (5), pp. 844-852. Date of Electronic Publication: 2022 Jun 24.
DOI:	10.1016/j.gastha.2022.06.002
Abstrakt:	Background and Aims: Recent evidence suggests that the gut is an additional target for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, whether SARS-CoV-2 spreads via gastrointestinal secretions remains unclear. To determine the prevalence of gastrointestinal SARS-CoV-2 infection in asymptomatic subjects, we analyzed gastrointestinal biopsy and liquid samples from endoscopy patients for the presence of SARS-CoV-2. Methods: We enrolled 100 endoscopic patients without known SARS-CoV-2 infection (cohort A) and 12 patients with a previous COVID-19 diagnosis (cohort B) in a cohort study performed at a regional hospital. Gastrointestinal biopsies and fluids were screened for SARS-CoV-2 by polymerase chain reaction (PCR), immunohistochemistry, and virus isolation assay, and the stability of SARS-CoV-2 in gastrointestinal liquids in vitro was analyzed. Results: SARS-CoV-2 ribonucleic acid was detected by PCR in the colonic tissue of 1/100 patients in cohort A. In cohort B, 3 colonic liquid samples tested positive for SARS-CoV-2 by PCR and viral nucleocapsid protein was detected in the epithelium of the respective biopsy samples. However, no infectious virions were recovered from any samples. In vitro exposure of SARS-CoV-2 to colonic liquid led to a 4-log-fold reduction of infectious SARS-CoV-2 within 1 hour ( P ≤ .05). Conclusion: Overall, the persistent detection of SARS-CoV-2 in endoscopy samples after resolution of COVID-19 points to the gut as a long-term reservoir for SARS-CoV-2. Since no infectious virions were recovered and SARS-CoV-2 was rapidly inactivated in the presence of colon liquids, it is unlikely that performing endoscopic procedures is associated with a significant infection risk due to undiagnosed asymptomatic or persistent gastrointestinal SARS-CoV-2 infections. (© 2022 Published by Elsevier Inc. on behalf of the AGA Institute.)
Databáze:	MEDLINE
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