Fabrication of a Tailored, Hybrid Extracellular Matrix Composite.

Autor: Setiawati A; Department of Chemistry and Institute of Biological Interfaces, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea.; Department of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea.; Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta, 55281, Indonesia., Jeong S; Department of Chemistry and Institute of Biological Interfaces, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea., Brilian AI; Department of Chemistry and Institute of Biological Interfaces, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea., Lee SH; Department of Chemistry and Institute of Biological Interfaces, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea., Shim JG; Department of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea., Jung KH; Department of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea., Shin K; Department of Chemistry and Institute of Biological Interfaces, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, Republic of Korea.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2022 Sep; Vol. 22 (9), pp. e2200106. Date of Electronic Publication: 2022 Jul 15.
DOI: 10.1002/mabi.202200106
Abstrakt: The extracellular matrix (ECM) is a network of connective fibers that supports cells living in their surroundings. Native ECM, generated by the secretory products of each tissue's resident cells, has a unique architecture with different protein composition depending on the tissue. Therefore, it is very difficult to artificially design in vivo architecture in tissue engineering. In this study, a hybrid ECM scaffold from the basic structure of fibroblast-derived cellular ECMs is fabricated by adding major ECM components of fibronectin (FN) and collagen (COL I) externally. It is confirmed that while maintaining the basic structure of the native ECM, major protein components can be regulated. Then, decellularization is performed to prepare hybrid ECM scaffolds with various protein compositions and it is demonstrated that a liver-mimicking fibronectin (FN)-rich hybrid ECM promoted successful settling of H4IIE rat hepatoma cells. The authors believe that their method holds promise for the fabrication of scaffolds that provide a tailored cellular microenvironment for specific organs and serve as novel pathways for the replacement or regeneration of specific organ tissues.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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