The gradient-like separation and reduced running time with Tris-Tricine-HEPES buffer for SDS-PAGE.

Autor: Dumut DC; Department of Experimental Medicine, Faculty of Medicine, McGill University, Montreal, QC, Canada., Garić D; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Centorame A; Department of Experimental Medicine, Faculty of Medicine, McGill University, Montreal, QC, Canada., Radzioch D; Department of Experimental Medicine, Faculty of Medicine, McGill University, Montreal, QC, Canada. Electronic address: danuta.radzioch@mcgill.ca.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2022 Sep 15; Vol. 653, pp. 114789. Date of Electronic Publication: 2022 Jun 20.
DOI: 10.1016/j.ab.2022.114789
Abstrakt: Tris-Glycine-SDS is the most commonly used running buffer for SDS-PAGE. Relatively long running times, poor resolution of small molecular weight proteins and excessive heat at higher voltages impede its utility for high throughput downstream applications such as western blot. Here we describe a protocol for gradient-like simultaneous separation of small (<10 kDa) and large (>400 kDa) proteins in a single percentage polyacrylamide Tris-Acetate gel using a novel running buffer composed of Tris, Tricine and HEPES.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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