Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction.

Autor: Oshiro S; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Mizutani N; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Tada T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Sekiguchi JI; Microbiology Research Division, Kohjin Bio Co., Ltd., Saitama, Japan., Takahashi M; Aotearoa Co., Ltd., Tokyo, Japan., Kirikae T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
Jazyk: angličtina
Zdroj: International dairy journal [Int Dairy J] 2022 Oct; Vol. 133, pp. 105436. Date of Electronic Publication: 2022 Jun 10.
DOI: 10.1016/j.idairyj.2022.105436
Abstrakt: Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1-1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa, and omicron, which contain 10, eight, seven, and over 30 mutations in the spike protein, respectively. We investigated whether bovine whey IgG enriched fraction contains antibodies against spike proteins of these variants, specifically recombinant partial length spike proteins (aa 177-512, aa 509-685, aa 177-324, aa 250-410 and aa 387-516) of these variants. Direct enzyme-linked immunosorbent assays revealed bovine whey IgG enriched fraction contained antibodies against all recombinant spike proteins of these variants with highest reactivity against aa 177-512 region of omicron spike protein. These results indicate bovine whey IgG enriched fraction contains antibodies against spike proteins of several SARS-CoV-2 variants, including omicron.
(© 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: